摘要
利用亚麻NCBI数据库中的7941条亚麻EST序列进行SSR的筛选,共发现222个SSR,占整个EST数据库的2.73%,其中三核苷酸重复单元的EST-SSR占总SSR的72.1%,二核苷酸和四核苷酸二者出现的频率基本相近,分别占总SSR的14.4%和13.5%.AGAA是四核苷酸中的优势重复类型,占四核苷酸重复类型的67.67%.设计的21对EST-SSR引物中有18对在10个亚麻材料中有扩增产物,占设计引物的85%,有14对产物条带比较清晰并具有多态性.基于SSR标记进行聚类分析,可将10个亚麻材料划分为3个组.本研究建立的亚麻SSR标记,为亚麻遗传多样性鉴定、分子作图等研究提供了一种有效的分子标记系统.
In this study,222 SSR from 7941 ESTs in NCBI are obtained,representing 2.73% of total ESTs. Trinucleotide repeats, accounting for 72.1 % of EST-SSRs, are dominant. Di- and Tri-nucleotide repeats are similar with frequency,accounting for 14, 4% and 13.5% of SSRs,respectively. AGAA is the most frequent motif,about 67.67% in tetranucleotide repeats. 18 pairs from 21 designed primer pairs of EST-SSRs show the amplification under a suitable PCR system in 10 flax germplasm. 14 primers amplify clear bands with high SSR polymorphism Based on SSR markers,dendrogram analysis could divide 10 flax germplasm into 3 groups. The developed Flax SSR markers in this study could provide a useful molecular system to identify the genetic diversity and to construct a fingerprint map.
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第5期910-915,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
现代农业产业技术体系专项资金资助(nycytx-22)
甘肃省农业生物技术研究与应用开发项目(GNSW-2008-13)
甘肃省自然科学科学基金项目(ZS021-A25-034-N)
油料作物品种创新及产业化发展研究创新团队项目
