摘要
为了进一步开发利用现有亚麻EST-SSR资源,从NCBI公共数据库下载7 947条亚麻EST,去除低质量和冗余序列后,筛选得到含有SSR的序列共239条,检出率为3.0%。其中二核苷酸重复49条,共7个重复类型;三核苷酸重复160条,共41个重复类型;四核苷酸重复30条,共11个重复类型。根据不同的重复类型共设计65对EST-SSR引物,在20份材料PCR扩增检测结果表明,有52对引物有目的扩增产物,其中32对扩增条带清晰且具有多态性,占设计引物的49.2%,通过聚类分析可将20份亚麻材料划分为五大类,验证了EST-SSR分子标记在亚麻遗传多样性分析中应用的可行性。
A set of 7 947 EST sequences from NCBI were used in order to develop the resource of flax EST-SSR.After assembly 239 EST-SSRs had been obtained,representing 3.0% of total,including 49 Di-nucleotide of 7 repeat types,160 Tri-nucleotide of 41 repeat types and 30 Tetra-nucleotide of 11 repeat types.52 primer pairs from the 65 designed EST-SSR primers showed amplification by a suitable PCR system in 20 flax accessions.32 pairs amplify clear bands with high polymorphism,representing 49.2% of total designed primers.20 flax accessions could be divided into five groups by dendrogram analysis based on EST-SSR markers,and indentify the development of EST-SSR marker could be used for analyzing genetic diversity of flax.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2012年第4期74-79,共6页
Journal of Northeast Agricultural University
基金
黑龙江省博士后留省工作基金(LBH-Q05035)
