摘要
目的：建立多波长同时测定3种獐牙菜属植物中6种活性成分含量的高效液相色谱方法。方法：采用Hypersil BDS色谱柱（4．6mm×200mm，5μm）；流动相为乙腈加．5％磷酸水溶液，梯度洗脱，流速为1．0mL·min；检测波长为240，274，325，334nm；柱温25℃。结果：在此色谱条件下，各组分在60min内均得到较好分离；獐牙菜苦苷、龙胆苦苷、去甲当药苷、雏菊叶龙胆苷、去甲基雏菊叶龙胆酮和雏菊叶龙胆酮分别在0．520～20．8，0．202—8．06，0．107—4．28，0．097～3．86，0．094～3．77，0．101～4．02μg线性良好，相关系数r均为0．9999，平均回收率分别为98．7％，98．1％，98．3％，98．8％，98．1％，98．6％，RSD均小于3．0％（n=6）。结论：本方法简单、快速、准确，重现性好，可为全面评价和控制獐牙菜属药材质量提供参考。
Objective： To develop an HPLC method for the quantification of six active components in three species （Swertia davidi, S. nervosa and S. mussotii） . Method： The determination was performed on a Hypersil BDS colunm （4. 6 mm ×200 mm, 5 μm）. Acetonitrile and 0. 5% phosphoric acid solution were used as the mobile phases with a gradient elution. The flow rate was 1.0 mL.min^-1. The UV detection wavelength was at 240, 274, 325 and 334 nm. The column oven temperature was at 25 ℃. Result： Six components were separated commendably in 60 minutes. The calibration curves of swertiamarin, gentiopicroside, norswertianolin, swertianolin, demethylbellidifolin and bellidifolin were in good linearity over the range of 0. 520-20. 8, 0. 202-8.06, 0. 107-4. 28, 0. 097-3.86, O. 094-3.77, 0. 101-4.02 μg, respectively （ r = 0. 999 9 ）. The average recoveries were 98.7% , 98. 1%, 98. 3 %, 98.8%, 98. 1% and 98.6%, respectively, and the RSD were less than 3.0% （n =6）. Conclusion： The method is accurate,simple and reproducible, and can be used to control the quality of Swertia
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第11期1384-1389,共6页
China Journal of Chinese Materia Medica
