摘要
目的:探讨瘤毛獐牙菜中分离所得的雏菊叶龙胆酮对糖尿病大鼠胰腺的保护作用机制。方法:将雄性Wistar大鼠随机分为正常组、模型组、雏菊叶龙胆酮(50 mg/kg)组,每组8只。模型组和雏菊叶龙胆酮组建立链脲佐菌素(STZ)诱导的糖尿病模型,雏菊叶龙胆酮组用雏菊叶龙胆酮进行干预,检测各组大鼠血清抗氧化因子超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性以及氧化代谢产物丙二醛(MDA)浓度,同时对胰腺组织HE染色并应用免疫印迹研究损伤相关因子NF-κBp65以及IκBα蛋白表达量的差异,分析雏菊叶龙胆酮对胰腺的保护作用。结果:雏菊叶龙胆酮能提高糖尿病大鼠血清CAT、SOD的活性(P<0.01),能降低MDA的浓度(P<0.01);胰腺HE染色显示雏菊叶龙胆酮能够减少糖尿病大鼠胰岛的萎缩,免疫印迹显示雏菊叶龙胆酮能增加胰腺组织内IκBα的表达,降低NF-κBp65蛋白表达。结论:雏菊叶龙胆酮可能通过胰腺NF-κB信号通路来减轻氧化应激水平,从而减轻STZ诱导的胰岛损伤,对胰岛起到保护作用。
Objective： To study the protective mechanism of bellidifolin separated from LiuMao ZhangYaCai （Swertia pseudochinensis Hara） for the pancreas of diabetic rats. Methods： The male Wistar rats were randomized into the normal group, the model group and bellidifolin group （50 mg/kg）, eight rats each group. The rats in the model group and bellidifolin group were established by STZ into diabetic models, bellidifolin group were intervened by bellidifolin. The protective effects of bellidifolin to the pancreas were analyzed by detecting the concentrations of oxidation metabolite malonaldehyde （MDA）, the activity of serum antioxidant factor SOD and catalase （CAT） of the rats in different groups, studying the difference between the contents of damage related factors NF-κBp65 and IκBα protein expressions after HE staining of the pancreatic island by western blotting method. Results： Bellidifolin could improve the activity of SOD and CAT （P〈0.01）, decrease the concentrations of MDA （P〈0.01）; HE staining of the pancreatic island showed that bellidifolin could relieve the atrophy of pancreatic island of diabetic rats, Western blotting demonstrated that bellidifolin could increase the expressions of IκBα in pancreatic tissue and decrease the expressions of NF-κBp65 protein. Conclusion： Bellidifolin could alleviate STZ-induced damage to pancreatic island and protect pancreatic island, which might ease oxidative stress level through pancreatic island NF-κB signal channel.
出处
《西部中医药》
2017年第11期40-43,共4页
Western Journal of Traditional Chinese Medicine
