摘要
目的:建立反相高效液相色谱法测定紫红獐牙菜药材中3种苷类成分含量的方法。方法:色谱柱为Alltimal C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(水中含0.05%磷酸),梯度洗脱,流速1 mL.min-1,检测波长254 nm,柱温30℃。结果:龙胆苦苷(gentiopicroside),芒果苷(mangiferin),8-O-β-D-吡喃葡萄糖基-1,5-二羟基-3-甲氧基酮(swertrianolin)分别在31.3～281.7,0.31～2.78,0.55～4.91μg内线性关系良好,相关系数分别为0.999 6,0.999 3,0.999 5;平均回收率分别为103.36%,101.42%,97.39%,RSD均小于3%(n=5)。结论:该方法操作简便,分离度好,为完善紫红獐牙菜药材的质量标准提供了依据。
To develop a RP-HPLC method for determination of three glycosides in Swertia punicea. Method： Chromatographic column： Alhimal C18 （4. 6 mm × 250mm, 5 μm）. Mobile phase： methanol-water （ including 0.05% H3PO4） , and gradient elution. Flow rate ： 1 mL·min^-1. Wavelength ： 254 nm. Column temperture： 30℃. Result： The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0. 31-2.78, 0. 55-4.91μg, （ r = 0. 999 6, 0. 999 3, 0. 999 5）. The average recoveries were 103.36%, 101.42% and 97.39% , with RSD less then 3% （n = 5）. Conclusion： It is a simple and sensitive meathod in controlling the quality of S. punicea.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第12期1184-1186,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30271590)
