摘要
应用水生生物体内实验方法，研究了２，４二硝基甲苯在鲤鱼肝肠提取物中的生物浓缩及代谢转化过程．半静态条件下，采用３种不同的暴露浓度，测定肝、肠组织的生物浓缩曲线，并同步对肝提取物进行ＨＰＬＣ检测．首次得到２，４二硝基甲苯还原代谢的两种产物：４氨基—２硝基甲苯和２，４二氨基甲苯，保留值分别为２３０５３ｍｉｎ及８７８９ｍｉｎ．以实验证明了峰形生物浓缩曲线是发生代谢转化的结论，且对鱼类的毒性预测研究也予以证实．
In vivo metabolism and bioconcentration of 2,4-dinitrotoluene(2,4-DNT) in liver and intestine of carps (Cyprinus Carpio L.) were investigated using semistatic system. Two metabolites of 2,4-DNT were separated from liver and identified as 4amino—2nitrotoluene(4A2NT) and 2,4-diamino toluene (2,4-DAT) on HPLC. Their retention times were 23.503 and 8.789 min, respectively. Such results demonstrated our inference that metabolism caused the declines in bioconcentration curves, and provided new experimental supports for research on toxicity of chemicals to fished.
出处
《环境科学学报》
CAS
CSSCI
CSCD
北大核心
1998年第3期303-307,共5页
Acta Scientiae Circumstantiae
基金
国家自然科学基金
关键词
二硝基甲苯
鲤鱼
代谢产物
水体污染
毒性
2,4-dinitrotoluene, carps, liver extract, metabolites, identification.
