摘要
以鲤鱼为被试生物，应用半静态方式，测定了全鱼、肝、肠各组织对2，4一二硝基甲苯（2，4－DNT）的生物浓缩曲线，全鱼得到包含下降段的峰形曲线．肝、肠均为包括2个连续出现的峰形趋稳态的曲线．全鱼的消除为双曲线形，肝部提取物经高压液相色谱鉴定，得到2种代谢物：4一氨基一2一硝基甲苯及二氨基甲苯．推测浓缩曲线下降段是由于发生了代谢作用．提出包括转化作用的生物浓缩模型，模拟了全鱼生物浓缩过程，得到与实际相符合的结果．
Bioconcentration curves of 2, 4-DNT in carps (whole fish, liver, intestine) wereinvestigated using semistatic systems. For whole fish, its curve could be described as agentle peak which began with a rise in concentration to steady state, then declined and reachedlower level followed by another steady state. For liver and intestine, their curves bothcontained two successive peaks. Bioconcentration factors (BCFs) of 2, 4-DNT in whole fish atthe first and second steady state were 9. 15 and 4. 15 respectively. By logarithmic ploting,two straight lines with different slopes 3. 6 and 0.1 d-1 were measured for elimination. Twometabolites were isolated by HPLC as 4-amino-2-nitrotoluene and 2,4-diaminotoluene, theirretention times were 23.1 and 8. 8 min respectively. A one-compartment model was set up tosimulate the uptake, in which biotransformation adhered to delayed enzyme-catalyticlogarithmic kinetics. Modeling curve was in accord with measured values.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1997年第10期1607-1610,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
2
4-二硝基甲苯
鲤鱼
生物浓缩
消除
代谢
2, 4-Dinitrotoluene(2, 4-DNT), Carps, Peaky bioconcentration curve
