摘要
目的建立液相色谱-串联质谱（LC-MS／MS）快速灵敏分析血液中茶碱的定性定量方法。方法将0．1mL血液加入0．9mL乙腈沉淀蛋白后，直接取上清液5皿进样。LC-MS／MS采用ESI（＋）模式，应用多反应监测（MRS）方法检测。结果本方法的液相分离良好，峰形优异，整个液相循环在3min内完成。采用0．1mL血液，茶碱和咖啡因在20-1000μg·L^-1时呈良好的线性关系，最低检测限（LDD）均为10μg·L^-1。结论所建立的方法简便、快速、特异性强、灵敏度高，可同时分析茶碱的代谢物咖啡因，足以满足临床毒物分析和法庭毒物分析的需要。
A/M To develop a rapid and selective method for the determination of theophylline in blood by liquid chromatography-tandem mass spectrometry（LC-MS/MS）. METHODS The drug was extracted from 0.1 mL blood using a protein precipitation with 0.9 mL acetonitrile and the supematants were analyzed by positive electrospray ionization （ ESI ＋ ）. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring （MRM） of two fragment ion transitions. RESULTS The chromatography was performed well and ran in 3 min. A linear relationship was used to
perform the calibration over an analytical range of 20 - 1 000 μg·L^-1. Limits of detection were found to be 10 μg·L^-1The method was simple and rapid, offering superior sensitivity and selectivity for theophylline and caffine simultaneously. CONCLUSION The method can be used successfully in clinical and forensic toxicology.
出处
《中国临床药学杂志》
CAS
2009年第3期139-142,共4页
Chinese Journal of Clinical Pharmacy
