摘要
目的:建立HPLC并结合常规分析方法对24批市售荜茇进行质量分析,对市售荜茇的质量进行综合评价。方法:采用薄层色谱法,用环己烷代替苯作展开剂对市售荜茇进行定性鉴别;按《中国药典》方法对水分、总灰分和浸出物进行测定;结合高效液相色谱外标法对其有效成分胡椒碱进行含量测定。结果:经优化的展开剂适合荜茇的薄层色谱鉴别;水分、总灰分均在《中国药典》规定限量之内,乙醇浸出物限度在8.11%～12.64%;胡椒碱在0.24～1.68μg·mL-1线性关系良好,平均含量为2.51%,平均回收率RSD=2.8%(n=6);结论:所建立的方法可对荜茇药材的质量进行综合评价,荜茇药材中胡椒碱的含量差异较大。
Objective： To establish HPLC combined with common analysis method for evaluating the quality of 24 batches of commercial Fructus Piperis Longi （FPL） and to provide evdience for the revision of quality standards of FPL. Methods： Cyclohexane as developing solvent instead of benzene in optimized TLC method . According to pharmacopeia （2005 edition）, water, total ash and ethanol extract of FPL were tested. Piperine as a marker constituent was determined by HPLC with external standard method. Results： The optimized developing solvent was suit for TLC identification of FPL. Both water and total ash were within limitation of pharmacopoeia and content of ethanol extract was 8.11-12.64%. The linear range for piperine was 0.24-1.68 μ g· mL^-1. Average content and recovery of FPL were 2.51% and 100.6% （RSD =2.8%） , respectively. Conclusion： The established method can be used in comprehensive evaluation of FPL and the contents of piperine in FPL which were purchased from different places were quite different.
出处
《中国现代中药》
CAS
2009年第5期35-38,共4页
Modern Chinese Medicine
关键词
荜茇
质量分析
薄层色谱鉴别
高效液相色谱测定
Fructus Piperis Longi
Quality analysis
TLC identification
HPLC determination
