摘要
目的建立蒙药那如 3中胡椒碱的含量测定方法。方法反相高效液相色谱法。采用Hyper silC18色谱柱 ;以甲醇 水 (V∶V =77∶2 3 )为流动相 ;流速为 1 0mL·min-1;柱温为室温 ;检测波长为3 43nm ;进样 2 0 μL;外标法计算含量。结果胡椒碱在 1 2～ 84mg·L-1内峰面积与对照品质量浓度呈良好线性关系 ,其回归方程为A =1 64× 1 0 5ρ -9 9× 1 0 3 ,r =0 9998,其平均回收率为1 0 1 4% ,RSD为 1 4% (n=9)。结论方法简便、准确 ,为那如 3的质量控制提供了方法。
Objective To determine the content of piperine in the Mongolian recipe Naru-3. Method The content of piperine in Naru-3 was determined by RP-HPLC with Hypersil C_ 18 column at room temperature.The mobile phase was methanol-water ( V∶V =77∶23) with a flow rate of 1.0 mL·min -1 and the injecting volume was 20 μL .The external standard was used and the wavelength of the detector was set at 343 nm .Results The linear range was 12～ 84 mg·L -1 ,and correlation coefficient was 0.999 8 .The average recovery and the relative standard deviation were 101.4% and 1.4%,respectively.Conclusion The method is simple,accurate,and can be used for the quality control of Naru-3.
出处
《沈阳药科大学学报》
CAS
CSCD
2004年第1期35-37,共3页
Journal of Shenyang Pharmaceutical University
