摘要
目的 通过合成纳米羟基磷灰石（hydroxyapatite，HA）以及体外实验观察其对牙本质小管的封闭效果，为纳米HA的临床应用提供依据。方法采用磷酸氢二铵[（NH4）2HPO4]和硝酸钙[Ca（NO3）2]合成纳米HA，扫描电子显微镜（scanning electron microscope，SEM）、透射电子显微镜（transmission electron microscope，TEM）、傅里叶变换红外光谱计（Fourier transform infrared spectrometer，FTIR）和X射线衍射仪（X—ray diffractometer，XRD）检测纳米HA。选取8颗健康第三磨牙制备24片牙本质薄片，按随机数字表分为酪蛋白磷酸肽钙磷复合体（casein phosphopeptideamorphous calciumphosphate，CPP—ACP）组、纳米HA组及空白对照组，每组8片；空白对照组不做处理，CPP—ACP组和纳米HA组每天分别用CPP-ACP和纳米HA处理牙本质薄片2次，7d后SEM观察牙本质表面。结果经SEM、TEM、FTIR和XRD检测，证实本项实验制备的HA为纳米HA；SEM观察纳米HA对牙本质小管的封闭效果明显高于CPP—ACP组和空白对照组。结论在体外条件下，与CPP—ACP相比，纳米HA可更有效地封闭暴露的牙本质小管。
Objective To synthesize nano-hydroxyapatite （nano-HA） and evaluate its effect on the occlusion of dentinal tubules in vitro, and therefore provide evidence for the clinical application. Methods （NH4）2HPO4 and Ca（NO3）2 were used to form nano-HA, which was characterized by scanning electron microscopy（ SEM ）, transmission electron microscopy （TEM）, Fourier transform infrared spectrometer （FTIR） and X-ray diffractometer（XRD） respectively. Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups ,which were control group, casein phosphopetide-amorphous calcium phosphate（CPP-ACP） group, and nano-HA group. After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days, the surface of slab dentin was observed using SEM. Results SEM, TEM, FTIR, and XRD tests showed that nano-HA was synthesized successfully. SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group. Conclusions In comparison with CPP-ACP, nano-HA could occlude dentinal tubules more effectively in vitro.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2009年第5期297-300,共4页
Chinese Journal of Stomatology
基金
湖北省科技攻关计划项目（2007AA301B28）
关键词
羟基磷灰石类
牙本质敏感
酪蛋白磷酸肽钙磷复合体
Hydroxyapatites
Dentin sensitivity
Casein phosphopeptide-amorphous calcium phosphate
