摘要
目的从定性和定量两方面研究不同粒径和形貌的羟基磷灰石(Hydroxyapatite,HA)对牙本质小管的堵塞作用及其耐磨性。方法选择人前磨牙40颗,以高速手机制取牙本质片并抛光,酸蚀后随机均分为4组:20 nm HA组;30 nm HA组;12μm HA组;空白对照组(不使用任何实验品)。各组分别用对应的材料刷洗牙本质片表面7 d。将牙本质片一分为二,一半扫描电镜观察;另一半进行刷牙磨耗实验后扫描电镜观察,应用Image-Pro Plus 6.0图像分析软件计算牙体质小管堵塞率。结果扫描电镜下见牙本质小管被颗粒状物堵塞,堵塞率达82%~96%,平均堵塞率为30 nm HA组(短棒状)>20 nm HA组(针状)>12μm HA组(球形)。牙刷磨耗实验后,扫描电镜下见牙本质表面沉积层光滑致密,仍有63%以上的小管被封闭。结论 30 nm短棒状HA、20 nm针状HA及12μm球形HA对牙本质小管均具有良好的堵塞作用及耐磨性。
Objective To study the blocking effect and abrasion resistance of hydroxyapatite(HA)with different particle sizes and morphologyies on dentinal tubules from the qualitative and quantitative aspects.Methods Dentin discs were etched and divided into4groups randomly:20nm HA group;30nm HA group;12μm HA group;control group(without any experimental treatment).Each group was brushed with correspondent materials for7days.Then,the dentin disk was divided into two parts,the one was detected by the scanning electron microscopy(SEM);the other was observed by SEM after toothbrush abrasion test.The Image?Pro Plus6.0image analysis software was used to calculate the plugging rate of dentinal tubules.Results SEM showed that the blockage is granular,the plugging rate of the dentinal tubules were about82%to96%.30nm HA group(short rod)range the first in the average plugging rate,followed by the20nm HA group(needles)and12μm HA group(spherical).After mechanical brushing for7days,SEM images showed that deposited layer in each group was smooth and compact,and more than63%of the tubules still had been blocked.Conclusion HA with different particle sizes and morphologies had good plugging effect and abrasion resistance on dentine tubules.The blocking effect of HA was affected not only by particle size but also by the morphology.
作者
杨剑珍
申晓青
刘成霞
徐平平
YANG Jian zhen;SHEN Xiao qing;LIU Cheng xia;XU Ping ping
出处
《口腔疾病防治》
2017年第5期288-293,共6页
Journal of Prevention and Treatment for Stomatological Diseases
基金
广东省自然科学基金(2014A030313330)
广州市科技计划项目(201707010199)
关键词
羟基磷灰石
牙本质过敏症
牙本质小管
纳米
脱矿
Hydroxyapatite
Dentine hypersensitivity
Dentine tubules
Nano
Demineralization
