摘要
[目的]通过比较正常孕妇与妊高症患者胎盘中Ghrelin及其受体GHS-R mRNA水平的变化,探讨Ghrelin及其受体与妊高症的发病是否相关。[方法]采用实时荧光定量RT-PCR技术检测了正常孕妇胎盘和妊高症患者胎盘中Ghrelin及其受体GHS-R mRNA水平相对表达量的变化。[结果]正常孕妇胎盘组织中mRNA水平与妊高症患者胎盘组织中mRNA水平无显著性差异,但Ghrelin受体GHS-R在两者之间的mRNA水平有显著性差异(P<0.05)。[结论]Ghrelin及其受体在妊高症患者和正常孕妇胎盘中表达的相对变化表明,Ghrelin受体缺乏可能成为妊高症发病的因素之一。
[Objective] The aim was to discussed the correlations of Ghrelin and its receptor （GHS-R） and pregnancy-induced hypertension syndrome by
comparing with nonnal pregnant women and pregnancy-induced hypertension syndrome patients in Ghrelin and its receptor （GHS-R） mRNA level changes.
[Method] Ghrelin and its receptor （GHS-R） mRNA expression changes were detected in normal pregnancy women and pregnancy-induced hypertension
syndrome patients by using real-time quantitative RT-PCR. [Result] The mRNA level was no significant difference between normal placenta of pregnant women and pregnancy-induced hypertension syndrome patients, however, the mRNA level of Ghrelin receptor （GHS-R） was significantly different（ P 〈 0.05）. [Conclusion] The result of expression relative changes of Ghrelin and its receptor in pregnancy-induced hypertension syndrome patients and normal placenta of pregnant women showed that the lack of Ghrelin receptor may be a risk factor of pregnancy-induced hypertension syndrome.
出处
《安徽农业科学》
CAS
北大核心
2009年第12期5367-5369,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30860201)
关键词
妊高症
胎盘
生长素
实时定量RT-PCR
Pregnancy-induced hypertension syndrome
Placenta
Ghrelin
Real-time quantitative RT-PCR
