摘要
目的:研究转录因子TWIST对胃癌细胞生物学行为的影响。方法:构建TWIST小干扰RNA,转染胃癌细胞系SGC7901,经G418筛选和Western blot鉴定TWIST低表达胃癌细胞系,MTT法测定细胞生长曲线,流式细胞仪技术检测细胞凋亡及细胞周期,Western blot法检测bax、bcl-2蛋白表达水平。结果:建立了TWIST低表达的胃癌细胞系SGC7901/pSi-TW,与亲本细胞及空载体转染细胞相比,SGC7901/pSi-TW细胞生长速度减慢、增殖指数降低、细胞凋亡增加、bcl-2表达下降、bax表达升高、bcl-2/bax比值降低。结论:TWIST在胃癌细胞的生长、细胞周期、凋亡中具有重要作用。
Objective ： To explore the effects of transcription factor TWIST on biological behaviors of gastric cancer cells. Methods ：The TWIST specific siRNA vector was constructed and transfected into gastric cancer cells SGC7901. The cell lines with knocked out expression of TWIST were screened and identified with G418 and Western blot. MTT assay, Flow cytometry and Western blot were used to detect the proliferation, apoptosis, cycle, bax and bcl -2 protein in gastric cancer cells respectively. Results：The cell lines SGC7901/pSi - TW with knocked out expression of TWIST were established. The growth of SGC7901/pSi - TW was slowed down. The proliferation index of SGC7901/pSi - TW was lower than that of control groups. The apoptosis rate was higher compared to control groups. The bcl - 2 and the bcl -2/bax ratio decreased in SGC7901/pSi -TW. Conclusion：TWIST may play an important role in the growth, cycle and apoptosis of gastric cancer cells.
出处
《现代肿瘤医学》
CAS
2009年第5期818-821,共4页
Journal of Modern Oncology
关键词
TWIST
转录因子
小干扰RNA
胃癌
TWIST
transcription factor
small interfering RNA
gastric cancer
