摘要
目的:研究原发性肝癌(PLC)组织中转录因子Twist,缺氧诱导因子-1α(HIF-1α)以及乙肝病毒x蛋白HBx)的表达及其相关性。方法:应用免疫组织化学SABC染色方法检测33例PLC组织及PLC合并肝硬化患者的癌旁组织、17例正常肝组织中Twist、HIF-1α以及HBx的表达,分析三者相关关系。结果:PLC组织中Twist、HIF-1α以及HBx的阳性表达率分别为93.9%,72.7%和81.8%。癌旁组织中Twist的表达率为54.5%。PLC与癌旁组织中Twist的表达有统计学意义(P<0.01)。正常肝组织中未见Twist表达。PLC组织中Twist分别与HIF-1α及HBx的表达呈显著正相关(γ=0.438,P=0.011;γ=0.445,P=0.009)结论:PLC组织中Twist、HIF-1α以及HBx的表达的上调,对肿瘤的发生、发展起着重要作用。
Objective：The study focused on detecting the expression of Twist, HIF-1α and HBx in primary liver cancer（PLC） and assessment their relativity.Methods：The expression of Twist, HIF-1α and HBx in 33 cases of PLC and thein paracancerous tissues were examinated with immunohistochemical location technique.Seventeen health control subjects were included in the study.Resulte：The incidence of Twist,HIF-1α and HBx expression was 93.9%,72.7% and 81.8% in 33cases of PLC,respectively.The positive percentage of Twist in paracancerous tissues was 54.5%.A significant difference was found between the expression of tumor and paracancerous tissures（P〈0.01）.The negative expression of Twist was found in normal liver tissue.The Twist expression was tightly correlated with HIF-1α（y=0.438 p=0.01）and HBx（y=0.445 p=0.009）in PLC.Conclusion：The expression of Twist,HIF-1α amd HBx in PLC were up-regulated.which play a vital role in progression of tumor.
出处
《中国现代普通外科进展》
CAS
2007年第1期37-39,44,共4页
Chinese Journal of Current Advances in General Surgery
基金
广西科学研究与计划项目(桂科能063006-5E)
