摘要
在pH值为0．65～1．10的HCl-NaAc缓冲溶液中，同多钨酸（IPT）与阿米卡星（AMK）形成离子缔合物，能引起共振瑞利散射（RRS）显著增强，并产生新的RRS光谱，其最大散射峰位于340nlTI，AMK质量浓度在1．0×10^-9～8．0×10^-8g／mL范围内与散射增强程度呈线性关系。建立了测定AMK的RRS新方法，检出限（3σ）为0．4×10^-9／mL。考察了体系的RRS和吸收光谱特征，优化了测定条件，考察了常见共存物质的干扰，方法具有良好的选择性。用于人血清中AMK的测定，结果满意。讨论了离子缔合反应和RRS增强机理。
In a pH 0.65~1.10 HCl-NaAc buffer solution,isopoly tungstic acid(IPT) was reacted with amikacin(AMK) to form an ion-association complex,which resulted in great enhancement of resonance Rayleigh scattering(RRS) and the appearance of a new RRS spectrum.The maximum scattering peak was located at 340 nm. The scattering intensity was proportional to the concentration of AMK in a range of 0.001×10-6~0.08×10-6 g/mL,based on which a new RRS method for the determination of AMK was established.The method exhibited a high sensitivity and the detection limit(3σ) was 0.4×10-9 g/mL.The RRS and absorption spectral characteristics and optimum reaction conditions of the system were discussed.Effects of coexisting substances were tested,and the results demonstrated that this method had a good selectivity.It was applied to the determination of AMK in human serum samples with satisfactory results.The reaction mechanism and reasons for RRS enhancement were discussed.
出处
《应用化学》
CAS
CSCD
北大核心
2009年第5期588-592,共5页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(20475045)
重庆市重点实验室(西南大学)(CSTC
2006CA8006)
重庆市科委自然科学基金计划(CSTC
2007BB1359)资助项目
关键词
阿米卡星
同多钨酸
共振瑞利散射
测定
amikacin,isopoly tungstic acid,resonance Rayleigh scattering,determination
