摘要
在弱酸性条件下，硫酸小诺霉素和丽春红S各自的共振瑞利散射十分微弱，两者相互作用后形成缔合物，导致体系的共振瑞利散射急剧增强，在597nm产生最大散射峰．研究了硫酸小诺霉素与丽春红S体系的共振瑞利散射光谱和吸收光谱特征、反应的适宜条件和共存物质的影响．实验结果表明，硫酸小诺霉素的浓度在0～7．2μg／mL范围内与散射强度（AIRRs）呈良好的线性关系，其回归方程为△I=2．038＋9．766C，相关系数0．9992，检出限为2．66ng／mL．本法简便、准确、灵敏、选择性和重现性好，已用于硫酸小诺霉素注射液和动物血清中硫酸小诺霉素的测定，结果满意．
In weak acidity condition, resonance rayleigh scattering （RRS） peaks of micronomicin sulfate （MIC）and ponceau S （PS） were very weak. However, MIC reacts with PS to form association particles, which enhance the RRS intensity greatly . The strongest RRS peak is at 597 nm. In this paper, the RRS spectra and absorption spectra of MIC-PS system were investigated, and the optimal reactive condition and the effect of the foreign substances for the determination of MIC were also established. The resonance scattering intensity at 597 nm is linear to the MIC concentration in the range of 0 to 7.2μg/mL, with a detection limit of 2.66 ng/mL. Its regression equation is AIRs=2. 038＋9. 766c,with a correlation coefficient of 0. 999 2. This method is highly selective, rapid, simple and repeatable and has been applied to the determination of MIC in animal serum and micronomicin sulfate injection, with satisfactory results.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第2期54-58,共5页
Journal of Southwest China Normal University(Natural Science Edition)
关键词
共振瑞利散射
硫酸小诺霉素
丽春红S
resonance rayleigh scattering
micronomicin sulfate
ponceau S
