摘要
以邻-三联苯为原料,经傅氏酰基化反应、酯酮缩合反应和氯磺化反应,合成了双功能螯合剂4,4′-二(1″,1″,1″-三氟-2″,4″-丁二酮-6″-酰基)-氯磺化邻-三联苯(BTBCT),经红外光谱、熔点、元素成份等表征分析,证实其结构;通过标记技术和荧光光谱分析确认BTBCT具有双向功能。BTBCT与BSA偶联,经UA仪监定后,再与Eu3+制成BSA-BTBCT-Eu3+。BTBCT可以作为新型固相TRFIA体系的双功能螯合剂。
To establish solid-phase time-resolved fluorescence immunoassay （TRFIA）, the bifunctional chelator BTBCT was synthesized with o-terphenyl as raw material by friedel-craft acylation, keton-ester condensed reaction and chlorosulfonation. The structure of synthesized BTBCT was verified by melting point, IR, 1 H NMR, and elemental analysis. BTBCT was conjugated to BSA and then successfully labeled with Eu^3＋ to form BSA- BTBCT-Eu^3＋. The BSA-BTBCT-Eu^3＋ showed a characteristic emission peak at 330nm by fluorescent analysis.BTBCT could be used as a new bifunctional chelator in solid-phase TRFIA.
出处
《标记免疫分析与临床》
CAS
2009年第2期105-108,共4页
Labeled Immunoassays and Clinical Medicine
基金
国家自然科学基金项目(No.30471606)
