摘要
目的：探讨烟草浸提液（smokeless tobacco extract，ST）对大鼠牙龈成纤维细胞（rat gingival fibroblasts，RGFs）在钛板上附着数目、形态、增殖的影响。方法：采用组织块法进行RGFs的原代培养，链霉菌抗生物素蛋白-过氧化物酶连结（SP）法进行细胞来源鉴定。不同浓度的ST作用于附着于钛板上的RGFs，MTF法检测钛板表面细胞的贴附、增殖情况．免疫荧光检测并分析钛板表面细胞铺展形态的变化。采用SPSS13．0软件包对数据进行单因素方差分析。结果：SP结果显示，RGFs波形丝蛋白染色阳性，角蛋白染色阴性，证明该细胞来源于中胚层。随着ST浓度的升高，RGFs在钛板表面的贴附、形态伸展、增殖呈下降趋势。ST作用组与阴性对照组间存在显著差异（P〈0．05）。结论：ST可抑制RGFs在钛板表面的贴附、形态伸展及增殖，提示吸烟可能影响口腔种植手术的远期效果。
PURPOSE： The study was designed to observe the effects of the smokeless tobacco extract （ST） on the attachment,morphology,structure and proliferation of rat gingival fibroblasts（RGFs） to titanium in vitro. METHODS： RGFs were obtained from explants of rat normal gingival tissues identified by immuuochemistry of vimentin and cytokeratin. by using tissue-explant technique.The origin of cells was RGFs to titanium were cultured in the presence of ST at various concentration,the attachment and growth of cells attached to titanium were measured by MTT method, immunofluorescence was used to detect and analyze the shapes of RGFs attached to titanium.Statistical analysis was performed using SPSS13.0 software package for one-way ANOVA. RESULTS： Immunochemical study showed that vimentin was expressed in RGFs while cytokeratin was negative,which indicated that RGFs were originated from mesoblastoma.With the increasing of ST concentration,the attachment,spreading shape and proliferation of RGFs in all groups decreased in a concentration-dependent manner.The difference between ST group and control group was statistically significant （P〈0.05）. CONCLUSIONS： ST can inhibit the attachment,spreading shape and proliferation of RGFs, suggesting that smoking may have influence on the long result of oral implant operation.Supported by Research Fund of Shandong Provincial Education Deoartment（Grant No.J04E26）.
出处
《上海口腔医学》
CAS
CSCD
2009年第2期173-177,共5页
Shanghai Journal of Stomatology
基金
山东省教育厅科技计划资助项目（J04E26）
关键词
钛
烟草
成纤维细胞
附着
增殖
Titanium
Tobacco
Rat gingival fibroblasts
Attachment
Proliferation
