摘要
目的探讨不同剂量的脑源性神经营养因子(BDNF)预先给药对大鼠局灶性脑缺血再灌注(I/R)后神经细胞凋亡的影响。方法用大鼠大脑中动脉线栓法(MCAO)建立局灶性脑缺血再灌注模型,BDNF采用侧脑室注射法给药。将30只雄性Wistar大鼠,随机分为5组:正常对照组(C组)、缺血再灌注组(I/R组)、BDNF预先给药低剂量组(L组)、中剂量组(M)、高剂量组(H);除C组外,其余各组分别行脑缺血1 h再灌注72 h后处死取脑皮层标本待检。采用TUNEL法观察大鼠脑缺血再灌注后脑皮层神经细胞凋亡的变化。结果与I/R组比较,BDNF预先给药各组神经细胞凋亡指数均明显减少(P<0.01);在BDNF预先给药各组中,以预先给药高剂量组的神经细胞凋亡指数最小(P<0.01)。结论不同剂量的BDNF预先给药均能明显减轻脑I/R后神经细胞凋亡,具有不同程度的脑保护作用。且随BDNF预先给药剂量的增加细胞凋亡数量进一步减少。
Objective： To study the effects of different doses of brain-derived neurotrophic factor （BDNF） pretreatment on apoptosis following focal cerebral ischemia and reperfu -sion in rats. Methods ： The model of middle cerebral artery occlusion （MACO） and reperfusion was set up. BDNF was injected into the lateral ventricle. Neuron apoptosis was identified by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling （TUNEL）. Thirty Wistar male rats were randomly divided into five groups, with six animals in each： sham operation group （group C）, ischemia-reperfusion group （I/ R）, BDNF pretreatment groups： L, M and H group. Except group C, the other group animals were sacrificed at the timepoint of cerebral ischemia lh and reperfusion 72h and then the brain cortex was takenfor detecting. Results： Compared with I/R group, the index of neuron apoptosis in BDNF pretreatment groups were decreased significantly （ P 〈 0. 01 ）. In all of the BDNF pretreatment groups, the neuron apoptosis index in brain cortex were lowest in group H （P 〈 0. 01 ）. Conclusion： Different doses of BDNF pretreatment can significantly decrease the number of apoptotic neuronal cells in cerebral cortex, has certain protection against focal cerebral ischemia-reperfusion injury. The number of neuron apoptosis decreases with the increase of the dose of BDNF pretreatment.
出处
《泰山医学院学报》
CAS
2008年第12期959-961,共3页
Journal of Taishan Medical College
