摘要
目的:研究人类白细胞抗原(human leucocyte antgen,HLA)-DRB1基因多态性与福建汉族人乙型肝炎肝硬化的遗传易感性关系.方法:以93例福建地区汉族人群乙型肝炎肝硬化患者为研究对象,以同一地区84例健康人为对照人群,采用DNA测序分型技术(sequencing-based typing,SBT)对HLA-DRB1等位基因精确分型,计算各组等位基因频率,对照人群等位基因分布进行Hardy-Weinberg遗传平衡检验,应用遗传统计方法进行关联分析,确定与乙型肝炎肝硬化相关的易感基因及基因型.结果:乙型肝炎肝硬化组HLA-DRB1*04等位基因频率明显高于对照组(OR=2.536,95%CI:1.292-4.978,P=0.0068),HLA-DRB1*1101等位基因频率明显低于对照组(OR=0.339,95%CI:0.119-0.964,P=0.0425);HLA-DRB104基因型在病例组与对照组之间分布差异具有统计学意义(OR=3.456,95%CI:1.553-7.692,c2=9.227,P=0.0024),乙型肝炎肝硬化与HLA-DRB04基因型剂量线性相关(OR=2.457,95%CI:1.274-4.737,c2=7.197,P=0.0073).结论:HLA-DRB1*04主型等位基因及其基因型可能是乙型肝炎肝硬化的易感基因,HLA-DRB1*1101等位基因可能为其抗性基因.
AIM： To investigate the association between HLA-DRB1 gene polymorphism and the genetic susceptibility to HBV-induced liver cirrhosis in Fujian Han population. METHODS： Genotyping of HLA-DRB1 gene was performed in 93 patients with HBV-induced cirrhosis and 84 healthy individuals using sequencing-based typing. The distribution of alleles in control group was checked by Hardy- Weinberg genetic equilibrium; association be- tween disease and frequencies of alleles were analyzed using genetic statistical methods to find the gene polymorphisms. RESULTS： The frequency of HLA-DRBI＊04 allele was significantly higher in patient group than in control group （OR = 2.536, 95%CI： 1.292-4.97, P = 0.0068）. However, the frequency of HLA-DRB1＊1101 allele was significantly lower in patient group than in control group （OR = 0.339, 95%CI： 0.119-0.964, P = 0.0425）. Signifi- cant difference was found in genotype of HLA- DRB1 04 between the case group and the control group （OR = 3.456, 95%CI： 1.553-7.692, 2 = 9.227, P = 0.0024）. There was significant dose-response relationship between liver cirrhosis and the genotype of HLA-DRB1 04 （OR = 2.457, 95%CI： 1.274-4.737,X^2=7.197, P=0.0073）. CONCLUSION： HLA-DRB1＊04 allele and HLA- DRB1＊1101 allele may be the susceptibility and resistant genes in the patients with liver cirrhosis respectively.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第9期886-890,共5页
World Chinese Journal of Digestology
基金
福建省自然科学基金资助项目
No.2006J0120~~
关键词
乙型肝炎
肝硬化
人类白细胞抗原
基因
频率
基因分型
Hepatitis B
Liver cirrhosis
Human leu-kocyte antigen
Gene frequency
Genotyping
