摘要
文章采用高效液相法测定唇膏中苏丹红染料(苏丹红Ⅰ～Ⅳ号)。试样中的苏丹红染料用氯仿∶乙醇(1∶1)提取出来后,经固相萃取净化,再用乙腈溶解定容,用HPLC进行测定。以乙腈—乙酸水溶液(95+5)为流动相,流速为1.0 mL/min,以Agilent TC C18柱(4.6 mm×250 mm,5μm)作为分析柱,进行分析测定。该方法检出限为10μg/kg,回收率为85%～105%。研究表明该方法准确而快速。
HPLC was applied to determination the amounts of sudan deys（sudan Ⅰ- sudanⅣ）in lipstick. Sudan dyes in the sample were extracted with solution（chloroform ： ethanol=1 ： 1）, and purified by solid phase extraction, then solubilized by acetonitrile. It was determined by HPLC. The Agilent TC C18 chromatographic column（4.6 mm×250mm,5μm）was used in the analysis. The mobile phase used was a mixture of acetonitrile and acetic acid solution with a ratio of 95 to 5 by volume. The flow-rate chosen was 1.0 mL/min Detection limit the new HPLC deys in lipstick of the method was found to be 10 μg/kg, and the result sobtained were in the range of 85 %-105 %. The results showed analysis method was both correct and fast, and the method was enough to be used in determinating the amounts of sudan deys in lipstick.
出处
《广东化工》
CAS
2009年第4期177-179,共3页
Guangdong Chemical Industry
