摘要
目的利用癌痛模型研究奇正消痛贴缓解癌性疼痛的作用及其机制。方法建立小鼠肺癌股骨癌痛模型,实验分治疗组、对照组、模型组和空白组。治疗组予奇正消痛贴0.75 g/kg,模型组和空白组予生理盐水0.5 mL/只,从造模第10天外用给药,连用5 d;对照组在造模第14天,单次6.498 mg/kg肌注硫酸四氢帕马丁。末次给药后测量各组小鼠的痛行为,包括机械性痛觉超敏和热刺激痛觉过敏;将小鼠股骨进行X线摄片,影像学评估骨破坏;用免疫组化法检测各组局灶皮肤组织肿瘤坏死因子α(TNFα)、内皮素-1(ET-1)、白介素-1β(IL-1β)水平及脊髓后角P物质受体(SP-R)、c-fos及GFAP阳性反应神经元的表达。结果造模第14天治疗组与模型组比较,对2g Von Frey纤维刺激的缩足反应百分率明显下降(P<0.01),热刺激痛阈时间明显延长(P<0.01);股骨X线显示,骨破坏程度较轻,放射学评分下降;局灶皮肤组织炎性介质及脊髓后角相关阳性反应神经元表达均明显减少。结论奇正消痛贴对癌痛小鼠治疗有效,能够减轻痛行为,延缓骨破坏,其镇痛机制可能与外周和中枢机制共同参与有关。
Objective To study the effect and mechanism of Qizhengxiaotong Emplastrum for relieving cancer pain by using cancer pain model. Methods Tbe model of cancer pain was established in mice with lung or femoral cancer. All mice were divided into the treatment group, control group, model group and blank group. The treatment group was given Qizhengxiaotong Emplastrum （0.75 g/kg）, and the model group and blank group were given normal saline solution （0.5 mL/each） externally for 5 days on 10th day after the establishment of model. The control group was given the intramuscular injection of sulfuric tetrahydropalmatine （6.498 mg/kg） on the 14th day ＇after model established. The pain behaviors including mechanical pain sensitivity and thermal hyperalgesia were measured in mice of all groups after the last drug administration. The X-ray radiography was used in mice on the femurs for the imaging evaluation of bone destruction. The immunohistoehemistr） method was used to detect the levels of tumor necrosis factor α（TNFα）, endothelin （ET-1） and interluking-1β （IL-1β） in focal skin tissue, and the expressions of positive neurons of the spinal dorsal horn including SP-R, c-los and GFAP in ＇all goups. Results On the 14th day the percent of paw withdrawal response induced by Von Frey fibers （2 g） was decreased significantly in the treatment group compared with that in the model group （P〈0.01）. The thermal analgesic latency was prolonged significantly in the treatment group than that in the control group （P〈0.01）. The result of X- ray radiography showed that bone destruction alleviated, radiological assessment decreased, and the inflammatory mediators of focal skin tissue and relevant positive neuron expressions reduced significantly. Conclusion Qizhengxiaotong Emplastrum is effective to mouse with cancer pain. and it can relieve pain behaviors and delay bone destruction. The analgesia mechanism may be related to the role played by peripheral and central mechanisms together.
出处
《北京中医药大学学报（中医临床版）》
2009年第2期1-5,共5页
Journal of Beijing University of Traditional Chinese Medicine
关键词
癌痛模型
奇正消痛贴
镇痛机制
外周
中枢
小鼠
cancer pain model
Qizhengxiaotong Emplastrum
analgesia mechanism
peripheral
central
mice
