摘要
目的:建立大鼠胫骨癌痛模型,观察电针和西乐葆对大鼠胫骨癌痛的缓解作用。方法:采用经皮穿刺技术将大鼠乳腺癌细胞Walker 256接种至雌性Wistar大鼠胫骨骨髓腔内,建立大鼠胫骨癌痛模型。大鼠造模后,采用电针和西乐葆灌胃治疗,观察其对大鼠骨肿瘤引起的机械性痛觉超敏的影响。结果:模型组大鼠后肢逐渐产生机械性痛觉超敏,接种后第16天胫骨近心端可见显著的肿瘤生长。单用电针或西乐葆5 mg/(kg.d)治疗大鼠,与模型组相比其机械性疼痛阈值差异无统计学意义;治疗后第22天和第26天,西乐葆10mg/(kg·d)组大鼠后肢机械性疼痛阈值较溶剂对照组提高(P<0.05);治疗后第10、18和23天,电针合用西乐葆5mg/(kg·d)组大鼠机械性疼痛阈值高于模型组,差异有统计学意义(P<0.05)。结论:电针与小剂量西乐葆具有协同作用,合用可以增强对大鼠胫骨癌痛的镇痛效果。
Objective： To establish a proper experimental model of bone cancer pain in rat for acupuncture research,and observe the pain-relieving effect of electroacupuncture（EA） and/or Celebrex on bone cancer pain in rats.Methods： The rat model of bone cancer pain was established by percutaneous direct puncture technique and inoculating the rat mammary gland carcinoma cells Walker 256 into tibial medullary cavity directly,and evaluated by detecting the bone tumor growth and mechanical allodynia.The effects of daily EA treatment and/or Celebrex treatment on the rat mechanical allodynia after intratibial Walker 256 inoculation was observed in this study.Results： Significant mechanical allodynia in ipsilateral hind paw and tumor growth in proximal end of tibial bone of rats in the untreated group were observed after intratibial Walker 256 inoculation.The mechanical allodynia thresholds in rats that received EA or 5 mg/（kg·d） Celebrex treatment showed no significant difference as compared with that of rats in the untreated group.However,the mechanical allodynia thresholds of rats in 10 mg/（kg·d） Celebrex group showed significant increase after 22-and 26-day treatment as compared with that in the methyl cellulose（MC） group.There was significant difference between rats with EA combined with 5 mg/（kg·d） Celebrex treatment and rats in the untreated group after 10-,18-and 23-day treatment.Conclusion： EA and 5 mg/（kg·d） Celebrex have synergistic effect on pain relieving and their combined use may enhance the analgesic effect on bone cancer pain.
出处
《中西医结合学报》
CAS
2008年第8期830-835,共6页
Journal of Chinese Integrative Medicine
基金
国家自然科学基金青年基金项目(No.30500678)
霍英东基金会高等院校青年教师基金(No.0246-05-FN360009)
复旦大学上海医学院青年骨干科研启动基金项目(No.07J03)
关键词
骨癌痛
电针
西乐葆
镇痛
大鼠
bone cancer pain
electroacupuncture
Celebrex
analgesia
rats
