期刊文献+

高效絮凝菌的鉴定及结构研究 被引量:1

Identification of Efficient Bioflocculant—Producing Strain and Study on Construction
下载PDF
导出
摘要 从活性污泥中筛选到一株高效絮凝剂产生菌RL-2。在1%CaCl2存在下,其发酵液对高岭土悬液具有较高的絮凝活性。根据形态及生理生化特征,利用16S rRNA部分序列分析确定该菌归属于Bacillus brevis。溶解性,茚三酮反应,Molish反应,电性试验及薄层色谱分析表明,RL-2所产高聚物为一阴离子型多糖,其结构单体为葡萄糖、半乳糖醛酸和甘露糖;由乌氏粘度法测得分子量大约为3.64×106Da。 The strain RL-2 which produced flocculating substance was screened from activated sludge.Their culture broths possessed the ability to flocculate kaolin suspension in the presence of 1% CaCl_2,which was identified to be a Bacillus brevis based on its morphological and physiological characteristics and the partial 16S rRNA sequencing method.The biopolymer produced by RL-2 was mainly composed of anionic polysaccharide,which was determined by solubility,Ninhydrin reaction,Molish reaction,electric test and TLC.It consisted of glucose,galacturonic acid and mannose with an approximate molecular size of 3.64×10~6Da.
作者 罗平 罗固源
出处 《城市环境与城市生态》 CAS CSCD 2006年第5期12-14,共3页 Urban Environment & Urban Ecology
基金 重庆大学骨干教师资助计划(716411049)
关键词 生物高聚物 正交实验 结构分析 短芽孢杆菌 biopolymer orthogonal test construction analysis Bacillus brevis
  • 相关文献

参考文献4

  • 1王曙光,李剑,高宝玉,岳钦艳,冯贵颖,姚利.产气肠杆菌(KLE-1)絮凝特性的研究[J].环境化学,2005,24(2):171-174. 被引量:17
  • 2刘进元等编著..分子生物学实验指导[M].北京:清华大学出版社,2002:228.
  • 3林加涵等主编..现代生物学实验 下[M].北京:高等教育出版社;施普林格出版社,2001:313.
  • 4李兆陇等编..有机化学实验[M].北京:清华大学出版社,2001:205.

二级参考文献11

  • 1王镇,王孔星,谢裕敏,姚茵丽.几株絮凝剂产生菌的特性研究[J].微生物学报,1995,35(2):121-129. 被引量:179
  • 2张彤,朱怀兰,林哲.微生物絮凝剂的研究与应用进展[J].应用与环境生物学报,1996,2(1):95-105. 被引量:60
  • 3Jang J H, Ike M, Kim S M et al. , Production of a Novel Bioflocculant by Fed-Batch Culture of Citrobacter sp.. Biotechnology Letters,2001, 23:593-597. 被引量:1
  • 4Kurane R, Hatamochi K, Kakumo T et al. , Production of a Bioflocculant by Rhodococcus Erythropolis S-I Grown on Alcohols. Biosci.Biotech. Biochem., 1994, 58 (2) : 428-429. 被引量:1
  • 5Tezuka Y, Cation-Dependent Flocculation in a Flavobacterium Species Predominant in Activated Sludge. Applied Microbiology, 1969,17 (2) : 222-226. 被引量:1
  • 6Sousa M J , Teixeira J A , Mota M, Difference in the Flocculation Mechanism of Kluyveromyces marxianus and Saccharomyces cerevisiae.Biotechnology Letters, 1992, 14 (3) : 213-218. 被引量:1
  • 7Attia Y A, Flocculation in Biotechnology and Separation Systems, in the Netherlands. Amesterdam: Elsevier Science Publishers B V,1987. 被引量:1
  • 8Haruhiko Yokoi, Tomomi Arima, Jun Hirose et al. , Flocculation Properties of Poly (T-glutamic Acid) Produced by Bacillus subtilis.Journal of Fermentation and Bioengineering, 1996, 82 (1) : 84-87. 被引量:1
  • 9胡筱敏,邓述波,牛力东,罗茜.一株芽孢杆菌所产絮凝剂的研究[J].环境科学研究,2001,14(1):36-40. 被引量:51
  • 10尹华,余莉萍,彭辉,张娜,叶锦韶.微生物絮凝剂JMBF-25的结构和性质[J].中国给水排水,2003,19(1):1-4. 被引量:47

共引文献16

同被引文献11

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部