摘要
以蒙古黄芪茎段为外植体,MS为基本培养基,设计不同浓度的IAA,NAA和6-BA等单因子和不同比例的细胞分裂素组合试验。结果表明,在芽的诱导增殖过程中,MS+NAA0 mg/L+6-BA1.0 mg/L的培养基效果最好;在诱导植株茎叶壮实高大增殖过程中,MS+NAA 0 mg/L+6-BA 0.3 mg/L的培养基效果较好,诱导率分别为89.2%和82.8%;生根培养过程中,MS+IAA2.0 mg/L的培养基效果较好,诱导生根率为55.9%。
Stalk segments of A stragcdus membranaceus Bge were cultured as explants on medium supplemented with plant hormone in different concentrations.The results showed that Murashige and Skoog （MS） medium supplemented with NAA 0 mg/L, 6-BA 1.0 mg/L was the most effective medium for the differentiation and multiplication of the shoots. In the process of proliferation for plant tall and sturdy stems and leaves, MS ＋ NAA 0 mg/L ＋6-BA 0.3 mg/L of the medium better. The induction rate was 89.2% and 82.8%.The optimum rooting medium was MS＋IAA 2.0 mg/L. The induction rate of root was 55.9%.
出处
《山西农业科学》
2009年第3期25-27,共3页
Journal of Shanxi Agricultural Sciences
基金
山西省农业科学院青年基金项目(YQN0507)
关键词
黄芪
茎段
组织培养
Astragalus membranaceus Bge
Stem
Tissue culture
