摘要
目的对Ｙａｍ１Ｂ细胞自发产生的一种可抑制人Ｔ、Ｂ细胞增殖的可溶性因子（Ｙａｍ－ＳＦ）进行研究。方法３Ｈ－ＴｄＲ掺入法进行淋巴细胞增殖抑制测定，ＥＬＩＳＡ法测定免疫球蛋白，间接免疫荧光法测定ＩＬ－２Ｒ，ＣＴＬＬ－２细胞３Ｈ－ＴｄＲ掺入法测定ＩＬ－２活性。结果Ｙａｍ－１Ｂ培养上清（Ｙａｍ－ＳＦ）经ｐｒｏｎａｓｅ水解消化而失活，经５６℃处理１０分钟，或在ｐＨ１０环境中处理６小时抑制活性丧失，在ｐＨ２．０环境中可保持其抑制活性。凝胶过滤试验表明，培养上清抑制活性分布在分子量４３０００～６７０００范围内，且ＴＧＦ－β抗体不能阻断其抑制活性。结论Ｙａｍ－ＳＦ可能是一种新的免疫调节因子，该因子可抑制人Ｔ细胞及Ｂ细胞的增殖，并抑制正常Ｂ细胞免疫球蛋白的产生，但对ＰＨＡ剌激的人淋巴细胞ＩＬ－２的产生以及ＩＬ－２受体（ＩＬ－２Ｒ）的表达无抑制作用。
Yam 1B,a human B lymphoblastoid cell line, spontaneously produced an immunoregulatory factor,which suppressed the proliferation of human T and B lymphocytes and inhibited immunoglobulin generation by normal B cells.The serumfree Yam 1B culture supernatant inhibited neither the expression of IL2 receptor nor the production of IL2 in the lymphocytes stimulated with PHA. The inhibitory activity of Yam 1B supernatant(YamSF) was inactivated at 56℃ for 10 min and at pH10.0 for 6h and abrogated by digestion with pronase, but remained at pH2.0. Gelfiltration column assay showed a peak of inhibitory activity in the fraction containing molecules of apparent MW of 43kD to 67kD. The inhibitory activity was not blocked by the antiTGFbeta antibody. These results indicate that YamSF appear to produce a novel immunoregulatory factor.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1998年第2期115-119,共5页
Chinese Journal of Microbiology and Immunology
基金
河南省自然科学基金
