摘要
目的:建立EB病毒转化B淋巴母细胞系(B-LCL),作为抗原提呈细胞(APC)提呈抗原肽,刺激短期培养的特异性T细胞活化并分泌IFN-γ,从而应用于T细胞表位鉴定中。方法:用B95-8细胞培养上清中的EB病毒转化肾综合征出血热(HFRS)患者PBMC,建立HFRS患者的B-LCL,以自身B-LCL为APC,加载抗原肽后,刺激短期培养的G9L特异的HFRS患者CD8+T细胞系,应用ELISPOT测定CD8+T细胞受到抗原肽刺激后产生IFN-γ的能力。结果:加载过抗原肽G9L或V15R的B-LCL可刺激G9L特异的CD8+T细胞活化并产生IFN-γ,而与G9L无同源序列的I15P则不能刺激G9L特异的CD8+T细胞活化。结论:B-LCL可作为非专职APC有效地将抗原肽提呈给特异性T细胞。
AIM： To establish Epstein-Barr virus （EBV）- transformed B lymphoblastic cell lines （B-LCL） to present peptides as antigen-presenting cells （APC） and stimulate short-cultured T cells secreting IFN-γ, by which the T cell epitopes are identified. METHODS： PBMCs from patients with hemorrhagic fever with renal syndrome （HFRS） were transformed using EBV from supernatant of B95-8 cells. ELISPOT assay was then employed to evaluate the IFN-γ production of short-cultured G9L-specific CD8^＋ T cells stimulated with peptide-pulsed autologous B-LCL cells. RESULTS： B-LCL pulsed with G9L or G9L-nested VI5R can stimulate GgL-specific CD8 ^＋ T cells producing IFN-γ, but not B-LCL pulsed with non-homologous I15P. CONCLUSION： B-LCL can efficiently and specifically present peptides to peptide-specific T cells as non-professional APC.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2009年第1期20-22,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30471625)
关键词
B淋巴母细胞
EB病毒转化
抗原提呈细胞
CTL表位
B lymphoblastic cell line
Epstein-Barr virustransformed
Antigen-presenting cell
CTL epitope
