摘要
目的探讨载脂蛋白E(apolipoprotein E,ApoE)基因第四外显子多态性与血管性痴呆(Vascular dementia,VD)的关系。方法通过DNA直接测序的方法检查30例VD和30例正常老人ApoE基因第四外显子的多态性。结果ApoE基因第四外显子存在112位点T/C多态和158位点C/T的多态,未发现新的单核苷酸多态性(single nucleotide polymorphism,SNP)。ApoEε4等位基因频率(11.7%,8.3%)及ε4/4,ε3/4,ε2/4基因型合并后其基因型频率(22%,16.7%)在VD和对照组之间无显著差异(x2=0.111,P>0.05;x2=0.739,P>0.05);VD组ε2等位基因频率与对照组相比差异不明显。结论结果提示ApoEε4等位基因不是本地区老年人群血管性痴呆的危险因素。
Objective To study relationship the forth exons ofApoE gene Polymorphism and Vascular dementia in Guizhou, China. Methods： the forth exons ofApoE gene in 30 VD patients and 30 normol people（control group） were screened with DNA sequencing method. Results： The allelic frequencies ofApoE ε 4 （11.7%, 8.3%） and genotype frequencies ofApoE ε 4/4, ε 3/4 and ε 2/4 in VD group （22%, 16.7%） were no significantly higher than those of control group （x^2=0.111, P〉0.05; x^2 =0.739, P〉0.05）. Conclusion： The ApoE ε 4 alleles might be not a genetic risk factor in the occurrence of VD in Guizhou, China.
出处
《当代医学》
2009年第7期71-72,共2页
Contemporary Medicine
