摘要
目的:探讨内皮细胞(endothelialcells,ECs)对血管平滑肌细胞(vascularsmoothmusclecells,VSMCs)整合素β1(β1-integrin)表达的影响,为研究血管壁细胞功能提供理论依据。方法:应用血管ECsVSMCs联合培养系统,以WesternBlot和细胞免疫荧光技术,检测与ECs联合培养12h后VSMCs的β1-integrin表达和分布变化。结果:与ECs的联合培养,能明显增强VSMCs的β1-integrin蛋白表达,同时诱导VSMCs表面β1-integrin的激活。结论:ECs可能通过调节VSMCs的β1-integrin表达,从而影响VSMCs的功能。
Objective： To study the effects of coculture with endothelial cells （ECs） on the expression of β1-integrin in vascular smooth muscle cells （VSMCs）. Methods： Human umbilical veins ECs and VSMCs were isolated. For coculture studies, ECs were grown to confluent on one side of a porous polyethylene terephthalate （PET） membrane. VSMCs were then plated on the opposite side of the membrane and cocultured for 12 hours. Next, the expression of β1-integrin of VSMCs was measured by Western blot and immunofluorescencc microscope. Results： VSMCs cocultured with ECs exhibited a significant increase in the expression of β1-integrin comparing to that of the control, i.e., VSMCs cultured alone. EC-coculture resulted in increased β1-integrin localization to the attached surface of VSMCs. Conclusions： The results suggest that induction of the expression ofVSMC β1-integrin by ECs, may partially influence VSMC function.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2009年第2期204-207,211,共5页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金资助项目(30770535
10732070)
