摘要
为提高杨梅资源的利用和活性成分的开发,运用四元二次回归旋转组合设计法及分光光度法探讨了杨梅鲜果花色苷的最佳提取工艺及杨梅花色苷粗提物清除DPPH自由基的效果。结果表明,曲面回归方程与实验结果拟合性好,优化工艺为提取温度35.34℃,液料比(v/w)11.02∶1,乙醇体积分数为68.09%,pH值2.72。在该条件下,杨梅花色苷的提取率为94.33%。将杨梅花色苷提取液通过大孔树脂进行分离,所制备的杨梅花色苷粗提物其清除DPPH自由基的效果优于EGCG,其IC50为0.1026mg·L-1。研究结果表明杨梅粗提物具有较强的抗氧化活性,其中花色苷等酚类物质可能为杨梅中重要的抗氧化成分。
In order to make full use of Chinese bayberry resource and exploit its active components, the optimal extraction technology for Chinese bayberry anthocyanins was studied by means of orthogonal rotational combination design, and the DPPH radical scavenging activity of the crude extract from fruits of Chinese bayberry was also researched. The results showed that regression equation fitted well with the experimental data, the optimum conditions of anthocyanins extraction were： ethanol concentration 68.09%, extraction temperature 35.34℃, pH value 2.72, ratio of solvent to material of 11.02：1 （v/w） and that the extraction rate of anthocyanins was up to 94.33%. The extract was further isolated with macro-porous resin and the crude extract was subsequently obtained by concentrating and freeze-drying. The DPPH radical scavenging activity of the crude extract from Chinese bayberry was stronger than that of EGCG, its IC50 value was 0.1026 μg·L^-1. The above results suggested that the crude extract of Chinese bayberry had very powerful antioxidant activity, and the polyphenols such as anthocyanins in crude extract may be the main antioxidant compounds of Chinese bayberry.
出处
《果树学报》
CAS
CSCD
北大核心
2009年第2期226-230,共5页
Journal of Fruit Science
基金
宁波市自然科学基金(No.2008A610067)
关键词
杨梅
黄酮
提取工艺
优化
抗氧化
Chinese bayberry
Anthocyanins
Extraction technology
Optimization
Antioxidant activity
