期刊文献+

柠檬酸盐对有机污水厌氧发酵产沼气的影响 被引量:5

Effect of citrate on anaerobic batch fermentation of organic wastewater for methane production
下载PDF
导出
摘要 将污水接种活性污泥后在厌氧、中温(37℃)和120 r/min条件下进行批培养,添加浓度为1.0 g/l柠檬酸钠时的产气量和产沼气对所消耗的葡萄糖的得率系数分别比对照提高38.5%和61.9%;但是糖平均消耗速率和最终pH值分别比对照下降了12.5%和7.1%。厌氧培养过程中的挥发性脂肪酸(VFA)变化趋势差异明显,对照VFA浓度在24 h达到最大值,此时其浓度是添加柠檬酸钠组VFA浓度的82%,之后逐渐降低。但是,添加组VFA含量随着培养的进行逐渐增大,VFA的平均积累速率和VFA对葡萄糖的得率系数分别比对照增加了232%和279%。柠檬酸钠的添加促进了葡萄糖向VFA的代谢合成,为甲烷合成提供了充足的底物,提高了葡萄糖转化甲烷的效率和沼气总量。 The effects of citrate on methane production during the treatment of organic wastewater were investigated in batch assays at 37℃ and 120 rpm. The results showed that the methane fermen- tation was stimulated. At a sodium citrate concentration of 1.0 g/L, the methane production and biogas yield in glucose consumed increased by 38.5% and 61.9%, respectively. However, the glucose con- sumption rate and the final pH value were 12.5% and 7.1% lower than those of the contrasted one, re- spectively. Further more,change of volatile fatty acids (VFA) during the course of anaerobic batch fermentation was obvious between citrate addition sample and the contrasted one. The maximal VFA concentration of the contrasted one reached at 24 h,which was 82% of that of the citrate addition sample , and then reduced gradually. However, the VFA content of the citrate addition sample in- creased along with cultivation process,and the VFA production rate and VFA yield in glucose con- sumption increased by 232% and 279% o The results suggest that citrate regulated the carbon flux to the synthesis of VFA, which facilitated the precursor supply of methane synthesis, and then en- hanced the efficiency of glucose utilization for methane and biogas production.
出处 《可再生能源》 CAS 北大核心 2009年第1期69-72,共4页 Renewable Energy Resources
基金 武汉市科学技术局攻关项目(200820212029) 湖北省教育厅重点项目(D20081401)
关键词 厌氧发酵 沼气 代谢 anaerobic batch cultivation biogas metabolism
  • 相关文献

参考文献13

  • 1WU JH,LIN CY.Biohydrogen production by mesophilic fermentation of food wastewater [J].Wat Sci Technol, 2004,49: 223-228. 被引量:1
  • 2GALAGAN JE,NUABAUM C.The genome of M. acetivorans reveals extensive metabolic and physiological diversity [J].Genome Res,2002( 12 ) :532-42. 被引量:1
  • 3STAMS AJ. Metabolic interactions between anaerobic bacteria in methanogenic environments [J].Antonie Van Leeuwen-hoek, 1994,66: 271-294. 被引量:1
  • 4STAMS AJ.Evidence for H2 and formate tormation during syntrophic degradation of bulvrate and propionale [J]. Anaerobe, 1995 ( 1 ) : 35-39. 被引量:1
  • 5任南琪 ... ..厌氧生物技术原理与应用[M],2004.
  • 6沈同 王镜岩.生物化学[M].北京:高等教育出版社,1998.207-208. 被引量:7
  • 7FRY B,ZHU T, DOMACH MM, et al. Characterization of growth and acid formation in a Bacillus subtilis pyruvate kinase mutant [J].Appl Environ Mierobiol, 2000,66 : 4045-4049. 被引量:1
  • 8BERRIOS-RIVERA SJ,BENNATF GN,SAN KY.The effect of increasing NADH availability on redistribution of metabolic fluxes in Eseherichia coli chemostat cultures [J].Metab Eng, 2002(4):230-237. 被引量:1
  • 9BERRIOS-RIVERA SJ, BENNATF GN,SAN KY. Metabolic engineering of Eseherichia coli: increase of NADH availability by overexpressing an NAD'-dependent formate dehydrogenase. [J].Metab Eng,2002(4): 217-229. 被引量:1
  • 10BOTT M.Anaerobic citrate metabolism and its regulation in enterobacteria [J].Arch Microbiol,1997.167 78-88. 被引量:1

共引文献6

同被引文献53

  • 1邢廷铣,方热军,谭支良,何烈华,陈惠萍.我国作物秸秆营养价值及其利用研究[J].农业现代化研究,1993,14(6):373-379. 被引量:7
  • 2沈同 王镜岩.生物化学[M].北京:高等教育出版社,1998.207-208. 被引量:7
  • 3王玉万 徐文玉.木质纤维素固体基质发酵物中半纤维素、纤维素和木质素的定量分析程序.微生物学通报,1987,14(2):81-84. 被引量:58
  • 4中国生物能源网.生物质能源的分类[EB/OL].http://www. bioenergy.cn/web/cyclopedia/HTML/cyclopedia 20071102010125 _ 61093. shtml,2007 - 11 - 02. 被引量:1
  • 5Wu JH, LIN CY. Biohydrogen production by mesophilic fermentation of food wastewater [J].Wat Sci Technol, 2004 (49) : 223-228. 被引量:1
  • 6GAIAGAN J E ,NUABAUM C.The genome of M. acetivorans reveals extensive metabolic and physiological diversity[J].Genome Res ,2002 (12) :532-542. 被引量:1
  • 7HENDRICKSON E L,KAUL R,ZHOU Y,et al. Complete genome seqyence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis [J].J bacteriol, 2004 (186) : 6956-6969. 被引量:1
  • 8STAMS A J. Metabolic interactions between anaerobic bacteria in methanogenic environments [J] .Antonie Van Leeuwen-hoek, 1994 (66) : 271-294. 被引量:1
  • 9STAMS A J. Evidence for H2 and formate formation during syntrophic degradation ofbutyrate and propionate [J].Anaerobe, 1995 ( 1 ) : 35-39. 被引量:1
  • 10RAUXA E,SCHUBER TB H L,WARRENA M J. Biosynthesis of cobalamin (vitamin B12) :a bacterial conundrum [J].Cell Mol Life Sci,2000(57):1880-1893. 被引量:1

引证文献5

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部