摘要
有机污水接种活性污泥后在厌氧、37℃和120 r·min-1条件下进行批培养,添加苹果酸盐(终浓度0.5 g·L-1)促进甲烷菌的三羧酸循环碳代谢和胞内的钴同化作用。结果表明,同化钴含量相对于对照提高了23.5%,这反映了以钴为中心离子的VB12的合成和以VB12为辅酶的甲基CoM还原酶的活性得到增强,并最终从产气效率上体现,其挥发性乙酸盐残留量比对照减少43.8%,而沼气总产量、TS和YP/V以及qp分别比对照提高了40.2%、15.2%、35.4%和22.4%,说明厌氧体系中添加苹果酸盐可以促进挥发性乙酸盐底物代谢流向终产物甲烷而非仅用于菌群繁殖,苹果酸盐是通过提高菌群的甲烷合成能力来提高产气,而非仅通过促进菌群生长的群体优势来提高产气。
The effect of malate on anaerobic batch methane fermentation of organic wastewater wase investigated at 37℃ and 120 r·min^-1. Adding malate at 0.5 g.L^-1 facilitated carbon metabolism of TCA cycle and assimilatory Co in methanogenesis, thus enhanced assimilatory Co concentration that was 23.5% higher than control, decreased residual volatile acetate by 43.8%, increased biogas, TS, YPN and qp by 40.2%,15.2%,35.4% and 22.4% ,respectively. It suggested that anaerobic wastewater fermentation with malate addition facilitated biogas production not only by increasing microorganism amounts, but also by enhancing the ability of microorganisms to biosynthesize methane.
出处
《湖北农业科学》
北大核心
2009年第9期2134-2136,共3页
Hubei Agricultural Sciences
基金
湖北省教育厅重点项目(D20081401)
武汉市科学技术局攻关项目(200820212029)
湖北工业大学博士启动基金项目(20070021)
关键词
厌氧发酵
沼气
代谢
苹果酸盐
anaerobic batch cultivation
biogas
metabolism
malate
