摘要
目的:探讨鼠肺缺血预处理后肺组织中热休克蛋白27(HSP27)的变化。方法:30只SD大鼠,随机分为缺血预处理组、对照组和假手术组,每组10只。缺血预处理组和对照组大鼠常规开胸后建立左上肺缺血再灌注模型,均遭受了缺血再灌注损伤,但缺血预处理组在缺血再灌注前给予缺血预处理干预,假手术组大鼠开胸后不予特殊处理。以免疫印迹方法比较不同组间肺组织中HSP27的表达差异。采用二维凝胶电泳分离缺血预处理组和对照组样本的总蛋白质,对相对分子质量介于20000～30000差异表达的蛋白质点进行基质辅助激光解吸电离飞行时间质谱分析。结果:缺血预处理组和对照组中HSP27的表达量均多于假手术组,但缺血预处理组中HSP27的表达量高于对照组。质谱分析显示有9个蛋白质斑点的表达量存在差异,其中3个斑点(点11、17和28)为同一个蛋白。结论:缺血预处理对肺脏缺血再灌注损伤有明显的改善作用,其作用可能与HSP27的高表达有关。
Aim ：To explore the protective effects of heat shock protein 27 （HSP27） in rat lung during ischemic preconditioning. Methods ：30 SD rats were randomly divided into preconditioning group（ group I）, control group （group C）and Sham operation group（ group S）. Each group contained 10 rats. Rat lungs in group I received ischemie preconditioning and the following ischemia-reperfusion insults, while those in group C suffered ischemia-reperfusion insults only. Rats in group S were not administered any other intervention after chest incision and lobe dissection. The expressed difference of HSP27 was compared by Western Blot. In addition, the total proteins of lung in group I and group C were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis. The differently expressed spots whose molecular weight vary from 20 000 - 25 000 were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry and database searching. Results： The expression of HSP27 both in group I and group C are more than that in group S. Compared with group C,the up-regulation of HSP 27 in group I is remarkably higher;HSP27 was posttranslationally modificated during the ischemic preconditioning and the ischemia-reperfusion process. Conclusion ： The HSP27 play a vital role in the protective procession of ischemic preconditioning on ischemia-reperfusion lung injury. Posttranslational modification of HSP27 is indispensable in this protective mechanism.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2009年第1期81-84,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
中国博士后科学研究基金资助项目2004036433
湖南省科技厅科研基金资助项目05sk3063
湖南省卫生厅科研基金资助项目B2004024
关键词
蛋白质组学
缺血预处理
肺保护
热休克蛋白27
proteomics
ischenfic preconditioning
lung protection
heat shock protein 27
