摘要
目的:观察胆总管短暂梗阻(TCBDO)对小鼠肾脏缺血再灌注(KIR)损伤的影响。方法:小鼠30只,采用胆总管结扎术建立TCBDO模型,于术后第0、1、2、3、4及5天测血清总胆红素(TB)、丙氨酸氨基转移酶(ALT)、肌酐(Cr)水平,于术后第3、4及5天测肾脏HSP27蛋白的表达。另取小鼠40只,随机分为4组,每组10只,分别给予胆总管结扎假手术、胆总管结扎术、胆总管结扎假手术+KIR造模术和胆总管结扎+KIR造模术,胆总管结扎术后第4天做KIR;缺血25min再灌注24h后,留取血样测血清Cr水平;留肾标本做PAS染色,观察形态学改变,MPO免疫组化染色分析肾中性粒细胞浸润情况,TUNEL法检测肾脏凋亡细胞。结果:TCBDO后小鼠血清TB和ALT水平快速上升,后迅速降低(P<0.001);血清Cr水平变化不大(P>0.05);肾脏HSP27蛋白表达水平增高(F=153.520,P<0.001)。与胆总管结扎假手术+KIR造模术组比较,胆总管结扎+KIR造模术组小鼠血清Cr水平(F交互=72.405,P<0.001)、肾组织病理评分(P<0.05)、中性粒细胞浸润程度(P<0.05)和凋亡细胞数(F交互=29.338,P<0.001)均降低。结论:TCBDO可能通过促进小鼠肾脏HSP27的表达,从而减轻肾KIR损伤。
Aim：To investigate the effects of transient common bile duct obstruction（TCBDO） on expression of heat shock protein 27（HSP27） and ischemia-reperfusion in kidneys（KIR） of mice.Methods：A total of 30 mice were performed common bile duct ligation（CBDL） to build TCBDO model, and the serum total bilirubin（TB）, alanine aminotransferase（ALT）,and creatinine（Cr） were measured at day 0,1,2,3,4 and 5 after CBDL, the expression of HSP27 protein in kidney tissue was detected at day 3,4,and 5 after CBDL. A total of 40 mice were divided into 4 groups randomly （10 in each group） and underwent CBDL sham surgery only,CBDL, CBDL sham surgery combined with KIR,and CBDL combined with KIR,respectively. KIR surgery was performed 4 days after CBDL.The serum Cr level was determined,morphological changes and neutrophil infiltration of the kidneys were observed by PAS and MPO immunohistochemical staining,respectively and cell apoptosis was detected by TUNEL.Results： After CBDL, the serum TB,ALT levels,but not Cr,changed significantly, and HSP27 protein expression in kidney tissue upregulated（F=153.520,P0.001）.In CBDL combined with KIR group,the serum Cr level decreased（F=72.405,P0.001）,and renal tissue injury score（P0.05）, neutrophil infiltration（P0.05） and apoptosis（F=29.338,P0.001） induced by KIR mitigated.Conclusion： TCBDO could attenuate KIR injury in mice probably through promoting the expression of HSP27 protein.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2012年第5期662-666,共5页
Journal of Zhengzhou University(Medical Sciences)
关键词
缺血再灌注
热休克蛋白27
胆总管梗阻
肾脏
小鼠
ischemia reperfusion
heat shock protein 27
common bile duct obstruction
kidney
mouse
