摘要
研究了不同浓度壬基酚对鲫鱼肝细胞增殖和抗氧化系统的影响.结果表明:各试验浓度壬基酚均能抑制鲫鱼肝细胞的增殖,其中高浓度的壬基酚(10-3mol·L-1)对细胞增殖的抑制作用极其显著,肝细胞形态发生明显改变;壬基酚破坏了鲫鱼肝细胞抗氧化系统的平衡,经壬基酚处理后的肝细胞超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性均受到抑制,而羟自由基的含量升高;壬基酚对原代鲫鱼肝细胞造成氧化损伤,引起培养液中丙二醛(MDA)含量升高.壬基酚诱导的氧化胁迫对原代鲫鱼肝细胞产生了一系列的体外毒性效应.
The study on the proliferation and anti-oxidative functions of Carassius auratus primary culture hepatecytes under effects of different concentration nonylphenol showed that at all test concentrations of nonylphenol, the proliferation of the hepatecytes was inhibited. High concentration （10^-3 mol · L^-1） nonylphenol had significant inhibitory effect, and induced an apparent morphological alteration of the hepatecytes. Nonylphenol upset the balance of hepatecytes anti-oxidative system through decreasing the activities of superoxide dismutase （SOD） and catalase （CAT） while increasing the hydroxyl free radical concentration. Nonylphenol also caused the oxidative damage on the hepatecytes, resulting in the increase of malondialdehyde （MDA） concentration in cell culture medium. The nonylphenol-induced oxidative stress engendered a series of in vitro toxic effects on the primary culture hepatecytes of C. auratus.
出处
《应用生态学报》
CAS
CSCD
北大核心
2009年第2期352-357,共6页
Chinese Journal of Applied Ecology
基金
上海市水产养殖重点学科建设项目(Y1101)
上海市水生生物重点学科建设资助项目(S30701)
关键词
壬基酚
肝细胞
鲫鱼
细胞增殖
抗氧化系统
nonylphenol
hepatecytes
Carassius auratus
cell proliferation
anti-oxidative system.
