摘要
采用聚丙烯酰胺凝胶电泳技术对鲶、黄颡鱼和乌鳢的肌肉、脑、心脏和肾4种组织的酯酶(EST)、乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)进行了比较分析。结果显示,鲶、黄颡鱼和乌鳢的4种组织中都存在EST、LDH和MDH同工酶,它们有明显的组织特异性:同种鱼的不同组织中存在不同的条带,不同组织的酶活性也不同。EST在鲶和黄颡鱼4种组织中都有很高活性,在乌鳢心脏中的活性最高。LDH和MDH在3种鱼肌肉和心脏的活性均明显高于脑和肾。
Comparative analysis on the isozyme patterns of LDH and MDH in four tissues （ muscle, brain, heart and kidney） from Siluru sasotus, Pseudobagrus fulvidraco and Ophiocephalus argus was carried out to prode into their distribution by Polyacrylamide Gel Electrophoresis （PAGE）. The result shows that the EST, LDH and MDH were found in these tissues and have obvious tissue - specific. In the same species, there are different isozyme bond among different tissue, and shows different activity in different tissues. EST have high activity among tissue from Siluru sasotus and Pseudobagrus fulvidraco, but only in heart from Ophiocephalus argus. LDH and MDH shows higher activity in both musculature and heart, followed is brain and kidney.
出处
《水生态学杂志》
北大核心
2008年第5期103-105,共3页
Journal of Hydroecology
基金
2005年南昌市科技局成果推广项目"鄱阳湖重要野生鱼类的种质资源生态库建设"
关键词
鄱阳湖
鲶
黄颡鱼
乌鳢
同工酶
Poyang lake
Silurus asotus
Pelteobagrus fulvidraco
Ophiocephalus argus
isozyme
