摘要
采用聚丙烯酰胺凝胶电泳(PAGE)及特异性组织化学染色技术,研究了黄颡鱼成体眼、心脏、肾脏、肝脏、肌肉及尾鳍等6种组织中的4种同工酶(LDH、MDH、SOD、EST)分化表达模式。结果表明,黄颡鱼的同工酶EST、SOD、MDH具有明显的组织特异性,LDH无明显的组织差异,并对同工酶的组织表达特异性的生理意义进行分析和讨论。
The patterns of expression in five multilous isozymic systems , esterase (EST),lcate dehydrogenase (LDH),malate dehydrogease (MDH), superoxide dismutase(SOD),were investigated in six differentiated tissue (eye?heart?kidney?liver?muscle and tail ) of Pelteobagrus fulvidraco . Isozymes were resolved by vertical polyacrylamide gel electrophoresis and then detected by specific histochemical staining respectively .The results show that isozymes in the adult Pelteobagrus fulvidraco exhibit a tissue specific expression apparently . The physiological significance was disscussed in relation to the extersive tissue specific distribution of the isozymes.
出处
《激光生物学报》
CAS
CSCD
2003年第4期274-278,共5页
Acta Laser Biology Sinica
关键词
同工酶
电泳
黄颡鱼
isozymes
electrophoresis
Pelteobagrus fulvidraco
