摘要
目的比较不同组合的冷冻保护剂对小鼠卵巢组织的影响,为人类卵巢组织玻璃化冷冻的保护剂选择提供实验依据。方法将20只小鼠的卵巢进行玻璃化冷冻,组织标本随机分为4组,新鲜的和冻融后的卵巢皮质分别进行光学显微镜观察、TUNEL实验和免疫组织化学分析。结果光镜观察发现各实验组的各级卵泡形态正常率均明显低于对照组,B组的始基卵泡形态正常率和初级卵泡形态正常率均高于A组和C组,差异有统计学意义(P<0.05)。A组和C组冻融后卵泡凋亡率、卵母细胞凋亡率和颗粒细胞凋亡率均高于B组,有统计学差异(P<0.05)。另外,B组卵泡Bax蛋白阳性表达率低于A组和C组,而B组Bcl-2蛋白阳性表达率高于A组和C组,差异具有统计学意义(P<0.05)。结论实验结果提示:与其它组合相比,EG与PROH的组合更适合小鼠卵巢组织的玻璃化冷冻。
Objective： To compare the effects of different combinations of cryoprotectants on mouse ovarian tissues after vitrification, in order to offer experimental gist for selecting the best combination of cryoprotectants. Method ： Ovarian tissue obtained from 20 monses was vitrified, and ovarian tissue slices were divided into 4 groups, follicles in ovarian cortical tissue were analyzed by light microscope, TUNEL assay and immunohistochemical examination respectively. Results： The percentage of morphologically normal follicles of the control group was significantly higher than those of all the experimental groups （ P 〈 0. 05 ）. The percentages of morphologically normal primordial and primary follicles in Group B were statistically higher than those in the Group A and Group C （ P 〈 0.05 ）. The percentages of apoptotic follicles, apoptotic oocytes and apoptotic granulosa cells in Group B were significantly lowerthan those in group A and Group C （ P 〈 0.05 ）. Percentage of follicles with Bax protein expression in Group Bwas significantly lower than those in the Group A and Group C （ P 〈 0. 05）, whereas, the percentage, follicles with Bcl - 2 protein expression in the Group Bwas higher significantly than those in the A Group A and Group C （ P 〈 0. 05）. Conclusions ： The results of the study indicate that the combination of EG and PROH is more suitable for vitrifying mouse ovarian tissue compared with other combinations.
出处
《中国优生与遗传杂志》
2009年第2期106-108,F0003,共4页
Chinese Journal of Birth Health & Heredity
关键词
组合
冷冻保护剂
卵巢组织
玻璃化冷冻
卵泡
Combination
Cryoprotectant
Ovarian tissue
Vitrification
Follicle
