摘要
目的探讨玻璃化冷冻法保存小鼠卵巢组织对卵泡内卵母细胞和颗粒细胞的影响。方法分别应用两种玻璃化冷冻方案(ED20和EE40)冷冻小鼠卵巢组织,常规组织学检测新鲜和复苏卵巢组织内卵泡形态,同时应用TUNEL方法观察冻融前后卵泡内卵母细胞和颗粒细胞凋亡情况。结果ED20组和EE40组卵泡存活率分别为78.99±5.99%和71.50±5.81%,明显低于新鲜卵巢组织。冻融组织卵泡凋亡率较冷冻前显著升高。ED20组、EE40组和新鲜对照组颗粒细胞凋亡的卵泡比例分别为8.30±2.14%、11.98±2.34%和4.95±1.62%,差异有显著性;而冷冻前后卵母细胞凋亡的卵泡比例无明显差异。结论玻璃化冻融过程对小鼠卵泡中颗粒细胞的损伤较大,而对卵母细胞的影响较小。
Objective ： To observe the effects of cryopreservation on oocytes and granulose cells in mouse ovarian tissues after vitrification. Methods： Ovarian tissues were vitrified with ethylene glycol （EG） combined with dimethyl sulphoxide （DMSO） or EG combined with egg yolk as cryoprotectants. Some of the non - frozen and frozen tissues were examined histologically, the others were detected opoptosis of oocytes and granulose cells. Results ： The viable percent of follicles in ED20 and EE20 were 78. 99 ± 5.99% and 71.50 ±5. 81% respectively, significantly lower than that in non -frozen tissues. In the frozen tissues, the percent of opoptosis follicles with opoptosis granulosa cells were 8.30 ±2. 14% and 11.98 ± 2. 34%, compared with 4.95± 1.62% in the non - frozen tissues （ P 〈0. 05 and P 〈0. 01 ）. However, the percent of opoptosis follicles with opoptosis oocytes in the frozen and non -frozen tissues were 1.24 ±0. 84, 2. 72 ±1.86 and 0.74 ±0. 85 respectively （ P 〉 0. 05 ）. Conclusion ： The granulosa cells, not oocytes in mouse follicles are affected by the cryopreservation after vitrification.
出处
《中国优生与遗传杂志》
2006年第11期102-103,F0002,共3页
Chinese Journal of Birth Health & Heredity
