摘要
本实验用ＰＣＲ、ＰＣＲ－ＳＳＣＰ银染方法对２４例血清ＨＢｓＡｇ（＋）患者肝癌及癌旁组织中ＨＢｘ基因表达和ｐ５３基因突变进行了检测。肝癌组织中ＨＢｘ基因表达率为１７％（４／２４），ｐ５３基因突变率为３３％（８／２４），癌旁正常组织和硬化组织中均无ＨＢｘ基因表达和ｐ５３基因突变。存在ＨＢｘ表达的肝癌组织中ｐ５３基因突变率为１００％（４／４），ｐ５３基因突变与ＨＢｘ基因表达具有相关性（Ｐ＜０．０１）。
PCR and PCR-SSCP were used to study p53 gene mutation and HBx gene expression in HCC tissues and tumor-adjacent tissues of serum HBsAg(+) with.The rate of p53 gene mutation was 33% in HCC tissues(8/24),the rate of HBx gene expression was 17%(4/24).Neither p53 mutaton nor HBx gene was founded in tumor-adjacent tissues.The rate of p53 gene mutation was 100%(4/4) in tumor tissues with HBx gene.p53 gene mutation was congratulated with HBx gene expression.It was suggested that the mechanism of p53 mutation was associated with intergrated HBx gene.
出处
《临床肝胆病杂志》
CAS
北大核心
1998年第2期83-85,共3页
Journal of Clinical Hepatology
