摘要
从甲醛固定的成人动脉粥样硬化斑块组织中提取DNA,用PCR扩增p53基因片段,将扩增的p53基因片段亚克隆至SK载体内,对扩增后的片段进行核苷酸序列分析。结果发现,动脉粥样硬化斑块中p53基因内第8外显子第272位密码子的T变为C。免疫组化分析观察到斑块内有p53基因突变蛋白的表达。提示p53基因突变可能与动脉粥样硬化的细胞增殖异常有关。
DNA was extracted from aldehydride fixed human atherosclerotic plaques. By the method of PCR, P53 gene fragments were amplified. Cloning these fragments into SK vector and sequencing them, we found that p53 gene T^(272) changed into C^(272). Immunohistochemistry showed that in plaques,the mutatant protein expressed. These results indicated that p53 gene mutation may be related with cell proliferation of atherosclerosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
1993年第1期1-3,共3页
Chinese Journal of Arteriosclerosis
