摘要
目的:比较瘢痕疙瘩成纤维细胞与正常皮肤成纤维细胞在增殖-凋亡方面的差异。方法:标本选取手术瘢痕疙瘩与正常皮肤各10例,分别用HE染色,增殖细胞核抗原(PCNA),末端脱氧核苷酸转移酶介导的末端标记技术法(TdT-Mediated dUIP Nickendlabeling,TUNEL)以及检测Caspase-3蛋白表达法来比较瘢痕疙瘩与正常皮肤在增殖一凋亡方面的差异。结论:与正常皮肤相比,瘢痕疙瘩增殖明显增加,凋亡明显减弱,这可能与其形成机制及浸润性生长有关。
Objective：To Compare the differences in the level of proliferation and apoptosis in keloid fibroblasts and normal skin fibroblasts. Methods： Keloid samples（N= 10） and normal skin samples（N= 10） were examined by HE staining, PCNA, TUNEL, and Caspase - 3 protein expression to compare the differenees in the level of proliferation and apoptosis. Conclusion：Compared with normal skin, keloid shows significantly increase in proliferation and decrease in apoptosis, which related in its formation mechanism and invasive growth.
出处
《激光杂志》
CAS
CSCD
北大核心
2008年第6期89-90,共2页
Laser Journal
基金
国家自然科学基金资助项目(编号:30572394/C03050302)
重庆市卫生局基金(05-2-049)
