摘要
目的:研究电针对快速老化痴呆模型小鼠(SAMP8)海马神经元突触超微结构、神经细胞黏附分子(NCAM)、多聚唾液酸转移酶ST8SiaII/IV的影响,从神经细胞黏附角度探讨电针治疗阿尔茨海默病(AD)的可塑性作用机制。方法:以SAMP8小鼠作为AD动物模型,电针"百会"、"涌泉"穴,1次/d,连续刺激21d。以Morris水迷宫测试小鼠学习记忆能力的变化;电镜观察海马神经元突触界面曲率、突触后致密物(PSD)厚度和突触间隙宽度的变化;以免疫组织化学、原位杂交方法检测海马神经元NCAM及其mRNA、ST8SiaII/IVmRNA的表达。结果:①模针组小鼠平均逃避潜伏期较模型组小鼠短(P<0.05);模针组小鼠在平台所在象限停留的时间(39.55±5.47s)较模型组小鼠(30.27±6.12s)长(P<0.05);②模针组突触后致密物厚度(76.928±25.236nm)较模型组(65.371±24.219nm)增厚(P<0.05);突触间隙宽度(25.941±6.217nm)较模型组(29.161±7.830nm)减小(P<0.05);突触界面曲率(1.083±0.049)较模型组(1.062±0.048)变化不明显(P>0.05);③与模型组比较,模针组小鼠NCAM及其mRNA、ST8SiaII/IVmRNA阳性表达明显增强(P<0.05)。结论:①电针能有效改善SAMP8小鼠学习记忆能力;②电针能有效调整海马神经元突触形态,使PSD增厚,突触间隙宽度变窄,促进突触形态可塑性的发挥;③电针改善SAMP8小鼠学习记忆能力的效应可能是通过诱导NCAM及ST8SiaII/IVmRNA的合成,促进神经细胞黏附,促进神经元突触形态可塑性来实现的。
Objective： To reveal the influences of electroaeupuncture（EA） therapy on the synaptic plasticity of hippoeampal neurons of senescence-accelerated mouse（SAMP8） and partly to explore the mechanism of EA on Alzheimer＇s disease（AD） based on neural cell adhesion. Method： SAMP8 was taken as animal model of AD. The treatment of acupuncturing on Baihui（Du20） and Yongquan（Kidl） with EA was applied once daily and lasted for 21d. Morris water-maze, electron-microscope and immunohistochemistry technique were used in study. Result：① Comparing with control group,the average escape latency in EA group decreased obviously（P〈0.05）, and the swimming time on platform quadrant in EA group（39.55±5.47s） was more than that in control group（30.27±6.12s）, （P〈 0.05）. ②Comparing with control group, structure of neural synapse in EA group was comparatively clear and com- plete,the thickness of post synaptic density（PSD） in EA group increased（P〈O.05）, the width of synaptic cleft in EA group decreased （P〈0.05） and the curvature of the synaptie interface in EA group had no obvious changes and just showed increasing-tendency （P〉0.05）.③Comparing with control group, the expression of NCAM and ST8SialI/IV of EA group increased significantly（P〈O.05）. Conclusion：EA therapy could improve the learning and memory abilities of SAMP8 mice, influence the morphological plasticity in hippocampal synapse, strengthen the information transmission of learning and memory,and these effects maybe related with the expression of NCAM and ST8SiaⅡ/IV enhanced.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2008年第12期1057-1060,共4页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金(NO.30472235)
四川省教育厅(2003C008)
关键词
阿尔茨海默病
突触可塑性
海马神经元
神经细胞黏附
电针
Alzheimer＇s disease
synaptic plasticity
hippocampal neuron
neural cell adhesion
electroacupuncture therapy
