摘要
目的体外实验研究丹参酮ⅡA对人肝内胆管癌细胞株HCCC-9810 Survivin基因的影响并探讨其与胆管癌细胞凋亡之间的关系。方法分别采用(0～10μg/ml)的丹参酮ⅡA作用人肝内胆管癌细胞72h后,通过反转录聚合酶链反应(reverse transcript polymerase chain reaction,PT-PCR)检测Survivin mRNA水平,采用蛋白质免疫印记法测定Survivin蛋白表达变化,通过MTT比色法观察其对细胞抑制影响,流式细胞仪行胆管癌凋亡测定。结果与对照组相比,丹参酮ⅡA以剂量依赖的方式抑制胆管癌细胞的生长,1～10μg/ml丹参酮ⅡA作用72h后,Survivin mRNA水平均受到显著抑制(P<0.05),5μg/ml以上剂量组抑制具有显著统计学差异(P<0.01)。结论在体外丹参酮ⅡA能够抑制人肝内胆管癌细胞株HCCC-9810 Survivin的表达,这可能是丹参酮ⅡA抑制其生长的机制之一。
Objective To investigate Significance and effect of tanshinone ⅡA on the expression of Survivin in human bile duct carcinoma cell line HCCC-9810 in vitro. Methods The human intrahepatic bile duct carcinoma cell line HCCC-9810 was treated with tanshinone ⅡA at various concentrations for 72 h. The effect of tanshinone ⅡA on the level of Survivin mRNA and the expression of Survivin protein were detected with the western blot analysis respectively. Cytotoxieity was evaluated by MTT assay. Apoptotic rate was quantified by flow cytometry （FCM）. Results Tanshinone ⅡA inhibited the growth of with 1～10μg/ml tanshinone AⅡ for 72 h, the level of gene expression of Survivin remarkably was downregulated（ P 〈 0.05 ） , the apoptotic index in radio salvia mihiorrhizae at the dose over 5 μg/ml group increased significantly（ P 〈 0. 01 ）. Conclusion Tanshinone ⅡA can induce the apoptosis of human bile duct carcinoma cell line HCCC-9810 Survivin in vitro, which may be related to the mechanism of growth inhibition of the human cell line.
出处
《肝胆外科杂志》
2008年第6期452-454,共3页
Journal of Hepatobiliary Surgery
基金
安徽医科大学附属省立医院2007年度基金资助项目
