摘要
目的体外实验研究丹参酮ⅡA对人肝内胆管癌细胞株HCCC-9810的生长抑制及诱导凋亡作用。方法分别采用(0～10μg/ml)的丹参酮ⅡA作用人肝内胆管癌细胞72h后,通过MTT比色法观察其细胞毒性,荧光显微镜、投射电镜检测细胞凋亡;流式细胞术(FCM)定量检测5μg/ml丹参酮ⅡA作用不同时间后的细胞凋亡率。结果丹参酮ⅡA以剂量依赖的方式抑制胆管癌细胞的生长。1～10μg/ml丹参酮ⅡA作用72h后,胆管癌细胞出现细胞皱缩、核染色质浓缩、核碎裂以及凋亡小体出现等特征性的形态学改变。5μg/ml浓度作用12、24、36、48、72h后的细胞凋亡率分别为(2.31±0.15)%、(3.02±0.36)%、(3.86±0.44)%,(6.75±0.60)%和(20.62±1.76)%与对照组(1.08±0.14)%比较均具有显著差异。结论在体外丹参酮ⅡA能够诱导人肝内胆管癌细胞株HCCC-9810凋亡,这可能是丹参酮ⅡA抑制其生长的机制。
Objective To investigate the effect of tanshinone ⅡA on growth and apoptosis in human Intrahepatic bile duct carcinoma cell line HCCC-9810 in vitro. Methods The human Intrahepatic bile duct carcinoma cell line HCCC-9810 was treated with tanshinone Ⅱ A at various concentrations for 72 h. Cytotoxicity was evaluated by MTr assay, appotosis-related alterations in morphology, fluorescence microscope and electron microscop are used to detee the apoptosis. Apoptotic tare was quantified by flow cytometry （FCM）. Results Tanshinone Ⅱ A inhibited the growth of with 1 - 10 μ/ml tanshinone Ⅱ A for 72h, HCCC-9810 cells apoptosis with nuclear chromatin concentration and fragmentation as well as cells shrinkage and the formation of apoptotic bodies were observed. FCM analysis showed hypodiploid peaks on histogram and the apoptotic rates at 5 μg/ml concentration for 12,24,36,48 and 72 h were （2. 31 ±0. 15）% 、（3.02 ±0. 36）%、（3.86 ±0.44）%、（6. 75±0. 60）% and （20. 62 ± 1.76）%. The control group was （1.08 ± 0. 14） %. Conclusion Tanshinone Ⅱ A can induce the apoptosis of human bile duct carcinoma cell line HCCC-9810 in vitro, which may be related to the mechanism of growth inhibition of the human cell line.
出处
《肝胆外科杂志》
2008年第5期380-382,共3页
Journal of Hepatobiliary Surgery
基金
安徽医科大学附属省立医院2007年度基金资助项目
