摘要
目的:探讨核心结合因子α1(cbfa1)在BMP-2调控体外培养的牙乳头细胞表达细胞外基质蛋白中的作用。方法:采用反义核酸技术,体外阻断培养的牙乳头细胞中cbfα1的表达,分别用RT-PCR、Western印迹等方法观察200ng/mLBMP-2作用6h后细胞中相关基质蛋白,碱性磷酸酶(ALP)、骨钙素(OC)、骨连蛋白(ON)、骨桥素(OPN)、骨涎蛋白(BSP)、牙本质基质蛋白1(DMP-1)以及牙本质涎磷蛋白(DSPP)的表达,采用SPSS11.0软件包对数据进行方差分析。结果:外源性BMP-2能明显上调牙乳头细胞中ALP、OC含量以及OPN、BSP和ON的表达,当反义阻断cbfα1的表达时,ALP、OC、OPN和BSP的表达显著降低(P<0.01)。结论:cbfα1参与了BMP-2调控体外培养的牙乳头细胞表达细胞外基质蛋白的信号转导过程。
PURPOSE： To evaluate the function of cbfα1 on BMP-2 signaling to extracellular matrix proteins in dental papilla cells in vitro. METHODS： RT-PCR and Western blot were performed to detect the expression of ALP, OC, ON, OPN, BSP, DMP-1 and DSPP in cultured dental papilla cells induced by 200ng/mL BMP-2 and/or down-regulated by cbfα1 antisense technology, the results were analysed with SPSS 11.0 software package. RESULTS： We found that the amount of ALP and OC and the expression of OPN, BSP and ON were upregulated significantly after the cells were treated with BMP-2. After transfected with antisense cbfα1, the cells downreguleted the expression of ALP, OC, OPN and BSP siguificantly（P〈0.01）. CONCLUSIONS： As a kind of transcription factor, cbfα1 could be an important tache in the BMP-2 signal networks controlling cells differentiation and mineralization.
出处
《上海口腔医学》
CAS
CSCD
2008年第6期611-615,共5页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30772416)~~
关键词
核心结合因子Α1
细胞外基质蛋白
反义核酸
Core binding factor α1
Extracellular matrix proteins
Oligo deoxynucleotide
