摘要
目的:探讨4种生长因子对体外培养的人牙乳头细胞中核心结合因子a1(core b ind ing factora1,cbfa1)表达的影响。方法:体外培养人牙乳头细胞,转染pcDNA3-cbfa1重组质粒,G418筛选建立稳定表达cbfa1的细胞模型PC-3,分别在正常细胞和PC-3细胞中加入不同浓度的BMP-2、TGF-β1、FGF-2、EGF,采用RT-PCR、W estern b lot、免疫组织化学的方法,观察这4种生长因子在人牙乳头细胞中对cbfa1基因表达、蛋白合成、蛋白表达部位的影响。结果:200 ng/mL BMP-2和50 ng/mL的FGF-2刺激正常人牙乳头细胞6 h后cbfa1的表达明显升高,而20和40 ng/mL的TGF-β1作用12 h,能抑制稳定转染cbfa1基因的细胞中cbfa1mRNA的表达;EGF则对人牙乳头细胞中cbfa1的表达没有显著影响。结论:BMP-2和FGF-2可以上调人牙乳头细胞中cbfa1的表达,TGF-β1对cbfa1的表达有抑制作用,EGF则对人牙乳头细胞中cbfa1的表达没有显著影响。
AIM ：To explicit whether the expression of cbfal is regulated by BMP -2, TGF - β1, FGF -2 and EGF in human dental papilla cells, METHODS：Human dental papilla cells were cultured in vitro and transfected with pcDNA3 -cbfal recombinant plasmids. After selected with G418 sulfate, a cell clone named PC -3, which could stably express the cbfal mRNA and protein,was proved by PCR and western blot, Then the expression of cbfal in normal cells and PC -3 cells treated with the four growth factors were detected by immunohistochemistry, western blot and PCR methods, RESULTS： The expression of cbfal were obviously upregulated in normal cells after 6 h treated with 200 ng/mL BMP- 2 or 50ng/mL FGF -2, and its in PC -3 cells was downregulated after 12 h treated with 20 or 40 ng/mL TGF - β1. EGF has no effect on the expression of cbfal in cells, CONCLUSION： In human dental papilla cells,cbfal could be upregulated by BMP- 2 or FGF -2 ,and downregulated by TGF- β1,
出处
《牙体牙髓牙周病学杂志》
CAS
2006年第6期303-306,共4页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(30271418)
关键词
CBFAL
牙乳头细胞
生长因子
core binding factor otl
dental papilla cells
growth factors
