摘要
目的建立青黛中靛蓝和靛玉红含量的测定方法。方法采用HPLC法定量分析,色谱柱为DiamonsilTM C18(250mm×4.6mm,5μm),流动相为甲醇-水(80:20),流速为1.0ml·min-1,柱温为30℃;靛蓝的检测波长为287nm,靛玉红的为292nm。结果靛蓝0.07～0.52μg(r=0.9996)、靛玉红0.02～0.20μg(r=0.9999)与峰面积的线性关系良好(r=0.9996),其平均回收率分别为100.9%(RSD=1.42%,n=6)、101.3%(RSD=1.87%,n=6)。结论所建方法可用于青黛中靛蓝和靛玉红的含量测定,为完善青黛的质量标准提供了依据。
OBJECTIVE To determine the contents of indigo and indirubin in Indigo Naturalis. METHODS The contents of indigo and indirubin were determined by HPLC, The chromatographic column was DiamonsilTM CI8 （250 mm × 4.6 mm,5μm）. The mobile phase was methanol -water（ 80 ： 20） . The flow rate was 1.0 ml.min^-1 with detection wavelngth of 287 nm for indigo and 292 nm for indirubin. RESULTS The linear correlation of the indigo was good in the range of 0. 07 - 0. 52 μg and the recovery was 100.9% with RSD of 1.42% （n= 6）. The Linear correlation of the indirubin was good in the range of 0. 02 -0. 20 μg and the recovery was 101.3% with RSD of 1.87% （ n = 6 ） o CONCLUSION The method can he applied to determine the contents of indigo and indirubin in Indigo Naturalis. It can ensure the stability of the quality control of Indigo Naturalis.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第6期714-715,共2页
West China Journal of Pharmaceutical Sciences
