摘要
目的采用HPLC同时测定人血浆中地西泮、氯硝西泮、三唑仑、艾司唑仑的浓度。方法血浆样品经氢氧化钠碱化后用乙酸乙酯萃取,氮气吹干,残留物用甲醇溶解后进样。采用Alltech-C18色谱柱,流动相为乙腈-甲醇-水(30:30:40),流速为1.0ml·min-1,检测波长为225nm。结果三唑仑0.05～10.00mg·L-1与峰面积的线性关系良好(r=0.9998),地西泮、艾司唑仑和氯硝西泮0.1～20.0mg·L-1与峰面积的线性关系良好(r=0.9997)。4种成分的方法回收率分别为96.8%、92.6%、97.7%、94.8%;日内和日间RSD均小于10%(n=5)。结论所建方法操作简便、快速、准确、灵敏度高、重复性好,适用于临床上人血浆中4种苯二氮类药物的同时定性、定量检测。
OBJECTIVE To establish an HPLC method for the simultaneous determination of four benzodiazepines （diazepam,clonazepam,trizolam and estazolam）. METHODS The drugs were extracted from the human plasma using ethyl acetate after basified with sodium hydroxide. The organic layer was evaporated to dryness under a nitrogen stream. The residue was reconstituted with methanol. Alhech-C18 column was used. The mobile phase consisted of acetonitril- methanol- water（30 ： 30 ： 40） at a flow rate of 1.0 ml. min ^-1 . The detection wavelength was set at 225 nm. RESULTS The linear range of triazolam was 0. 05 - 10.130 mg.L^ -1（ r = 0. 9998 ） , and which of the other drugs was 0. 1 - 20.0 mg. L^- 1 （ r = 0. 9997 ）. The average recovery of diazepam, clonazepam, trizolam and estazolam was 96. 8% , 92. 6% , 97.7% and 94. 8% respectively. The intra - day RSD and inter - day RSD were less than 10% （n =5）. CONCLUSION The method is simple, accurate, rapid and reproducible, and can be used in clinic determination of the four benzodiazepines in human plasma.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第6期699-701,共3页
West China Journal of Pharmaceutical Sciences
基金
宁夏自然科学基金(编号:NZ0558)
